Receptor-like kinases (RLKs) are essential for plant abiotic stress responses. Methylglyoxal (MG) is a cellular metabolite that is often considered to be a stress signal molecule. However, limited information is available about the relationship between RLKs and MG. Here, we addressed the function of a receptor-like kinase, OsASLRK, in the MG response and content in rice. A typical MG-responsive element (AAAAAAAA) exists in the promoter region of the OsASLRK gene. RTqPCR analysis indicated that the transcript level of OsASLRK was significantly increased by exogenous MG in a time- and dosage-dependent fashion. GUS staining also confirmed that the expression of OsASLRK in rice root was enhanced by exogenous MG treatment. Genetic analysis suggested that the Osaslrk mutant displays increased sensitivity to MG and it showed higher endogenous MG content under exogenous MG treatments, while OsASLRK-overexpressing rice plants showed the opposite phenotypes. Diaminobenzidine (DAB) staining, scavenging enzyme activities and GSH content assays indicate that OsASLRK regulates MG sensitivity and content via the elevation of antioxidative enzyme activities and alleviation of membrane damage. Therefore, our results provide new evidence illustrating the roles that receptor-like kinase OsASLRK plays in MG regulation in rice.

受体样激酶 (RLK) 对植物非生物胁迫反应至关重要。甲基乙二醛 (MG) 是一种细胞代谢物，通常被认为是一种应激信号分子。然而，关于 RLK 和 MG 之间关系的信息有限。在这里，我们解决了受体样激酶Os ASLRK 在水稻中 MG 反应和含量中的功能。OsASLRK基因的启动子区存在典型的 MG 响应元件 (AAAAAAAAA) 。RTqPCR 分析表明，外源性 MG 以时间和剂量依赖性方式显着增加OsASLRK的转录水平。GUS染色也证实外源MG处理增强了水稻根中OsASLRK的表达。遗传分析表明Osaslrk突变体显示出对 MG 的敏感性增加，并且在外源 MG 处理下显示出更高的内源 MG 含量，而OsASLRK 过表达的水稻植物显示出相反的表型。二氨基联苯胺(DAB) 染色、清除酶活性和 GSH 含量测定表明，OsASLRK通过提高抗氧化酶活性和减轻膜损伤来调节 MG 敏感性和含量。因此，我们的研究结果提供了新的证据，说明受体样激酶Os ASLRK 在水稻 MG 调控中的作用。