Brucine are the main constituents of Strychnos nux-vomica. Earlier reports have determined brucine shows anti-inflammatory, analgesic and excellent anti-tumor drug. Even though its anticervical cancer cells remains not clearly evaluated. So that, we hypothesized the anti-cervical cancer activity of brucine against the cervical (ME-180) cells. Brucine inhibited the inflammation, cell proliferation and promoted rate of apoptotic cell death ad reduced the mitochondrial potential, which is evidenced by respective (AO/EB, Rh-123, and PI) staining. Furthermore ELISA and real time PCR reaction determined that brucine were down regulated inflammatory (TNF-α, NF-kB, IL-6 & COX-2) cell proliferation (Cyclin D1) and apoptotic marker Bax, caspase-3, PI3K (phosphoinosital 3 kinase), AKT, mTOR (mammalian target of rapamycin) and over expression Bcl-2, associated death promoter. These findings were confirmed and finally suggested that brucine inhibited inflammation, cell proliferation and promoted the apoptosis through the down-regulation of PI3K/AKT/mTOR pathway. Taken together, these data were exhibited brucine as a good therapeutic agents for the prevention of anticancer cervical cancer drugs.

中文翻译：

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Brucine 通过调节 PI3K/AKT/mTOR 信号通路抑制炎症和细胞增殖促进宫颈癌细胞 (ME-180) 凋亡

Brucine 是Strychnos nux-vomica的主要成分. 较早的报道已确定马齿苋碱显示出抗炎、镇痛和优良的抗肿瘤药物。尽管其抗宫颈癌细胞仍未得到明确评估。因此，我们假设了马齿苋对宫颈 (ME-180) 细胞的抗宫颈癌活性。Brucine 抑制炎症、细胞增殖和促进凋亡细胞死亡率并降低线粒体电位，这由各自的（AO/EB、Rh-123 和 PI）染色证明。此外，ELISA 和实时 PCR 反应确定，马铃薯碱下调炎症（TNF-α、NF-kB、IL-6 和 COX-2）细胞增殖（细胞周期蛋白 D1）和凋亡标志物 Bax、caspase-3、PI3K（磷酸肌醇 3激酶）、AKT、mTOR（哺乳动物雷帕霉素靶点）和过表达 Bcl-2，相关死亡启动子。这些发现得到证实，并最终表明马钱子碱通过下调 PI3K/AKT/mTOR 通路抑制炎症、细胞增殖和促进细胞凋亡。综上所述，这些数据表明马钱子碱是预防宫颈癌抗癌药物的良好治疗剂。