Health benefits associated with high amylose starch have drawn scientific interest to elevate amylose content in wheat. Despite of several attempts, the elucidation of genetic factors behind high amylose biosynthesis remains unclear. We identified variation for amylose content in a bi-parental population (F_2:3), developed by crossing of high amylose donor ‘TAC 75’ and high yielding variety ‘WH 1105’ and report the first QTL map by Genotyping-by-sequencing. A stable QTL qhams7A.1 among 12 major effect QTLs was identified. Gene Ontology identified GBSSI, within qhams7A.1. High amylose donor carrying a novel missense mutation g.35767184 T > C in GBSSI led to mutant GBSSI_L539P. Subsequent analysis of mutant allele in a backcross population suggests the contribution of mutant allele to phenotype. The identified QTLs and GBSSI allele provide a useful resource for marker development and pre-breeding material for the trait.

与高直链淀粉相关的健康益处引起了科学兴趣，以提高小麦中的直链淀粉含量。尽管进行了多次尝试，但对高直链淀粉生物合成背后的遗传因素的阐明仍不清楚。我们通过高直链淀粉供体“TAC 75”和高产品种“WH 1105”杂交开发的双亲群体 (F _2:3 )鉴定了直链淀粉含量的变异，并通过基因分型测序报告了第一个 QTL 图. 在12个主要效应QTL中鉴定出一个稳定的QTL qhams7A.1。Gene Ontology在qhams7A.1 中鉴定了GBSSI 。高直链淀粉供体携带的新型错义突变g.35767184 T>下在GBSSI导致突变 GBSSI_L539P。随后对回交群体中突变等位基因的分析表明突变等位基因对表型的贡献。鉴定的 QTL 和 GBSSI 等位基因为标记开发和性状的预育材料提供了有用的资源。