This study evaluated the impact of the TLR7 Gln11Leu (rs179008) and TLR9 −1237 T/C (rs5743836) single nucleotide polymorphisms (SNPs) on susceptibility to placental infections and pregnancy complications in 455 Brazilian women. Demographic, socioeconomic, gynecological, and clinical characteristics of the women were collected. Placental tissues were sampled from pregnant women and human and viral DNA was extracted. Human alphaherpesvirus 1 (Herpes simplex virus type 1, HSV-1), Human alphaherpesvirus 2 (Herpes simplex virus type 2, HSV-2) and Human betaherpesvirus 5 (Human cytomegalovirus, HCMV) were detected by nested PCR. TLR9 and TLR7 SNPs were genotyped by PCR amplification of bi-directional specific alleles (Bi-PASA) and restriction fragment length polymorphism (RFLP), respectively. Infections at the time of birth were detected in 45.71 % of women. The presence of the TT genotype (recessive model) of the TLR7 SNP was associated with increased susceptibility to HSV-1 infection (O.R. = 2.23, p = 0.05). The presence of the C allele of the TLR9 SNP, in heterozygosis or homozygosis (dominant model), decreased the infection risk by HCMV (O.R. = 0.31, p-mod<0.05). The TT genotype (recessive model) of the TLR7 SNP was significantly associated (p < 0.05) with increased occurrence of pre-treated hypertension. The codominant model of the TLR9 SNP was significantly associated (p < 0.05) with reduced risk of hospitalization during pregnancy. In combination, the AA/CT (TLR7-TLR9) genotypes significantly decreased the risk of placental infection by HSV-1 and/or HSV-2 (O.R. = 0.47, p = 0.02), the susceptibility to all infectious agents considered in combination (O.R. = 0.4, p = 0.00), and the need of hospitalization (O.R. = 0.48, p = 0.02). In conclusion, TLR7 and TLR9 SNPs are potential modulating factors for the risk of placental infections and pregnancy complications.

本研究评估了TLR7 Gln11Leu (rs179008) 和TLR9 -1237 T/C (rs5743836) 单核苷酸多态性 (SNP) 对 455 名巴西妇女胎盘感染和妊娠并发症易感性的影响。收集了女性的人口统计学、社会经济、妇科和临床特征。从孕妇身上采集胎盘组织，提取人类和病毒 DNA。通过巢式 PCR 检测人 alphaherpesvirus 1 (Herpes simplex virus type 1, HSV-1), Human alphaherpesvirus 2 (Herpes simplex virus type 2, HSV-2) 和Human betaherpesvirus 5 (Human cytomegalovirus, HCMV)。TLR9和TLR7分别通过双向特异性等位基因 (Bi-PASA) 和限制性片段长度多态性 (RFLP) 的 PCR 扩增对 SNP 进行基因分型。45.71% 的女性在出生时检测到感染。TLR7 SNP的 TT 基因型（隐性模型）的存在与 HSV-1 感染的易感性增加相关（OR = 2.23，p  = 0.05）。在杂合或纯合（显性模型）中， TLR9 SNP的 C 等位基因的存在降低了 HCMV 的感染风险（OR = 0.31，p -mod <0.05）。TLR7 SNP的 TT 基因型（隐性模型）显着相关（p < 0.05) 治疗前高血压的发生率增加。TLR9 SNP的共显性模型 与怀孕期间住院风险的降低显着相关（p < 0.05）。组合起来，AA/CT ( TLR7 - TLR9 ) 基因型显着降低了 HSV-1 和/或 HSV-2 胎盘感染的风险 (OR = 0.47, p  = 0.02)，对所有感染因子的敏感性结合起来考虑 ( OR = 0.4，p  = 0.00）和住院需要（OR = 0.48，p  = 0.02）。总之，TLR7和TLR9 SNP 是胎盘感染和妊娠并发症风险的潜在调节因素。