A simple, reliable and reproducible high-performance thin-layer chromatography (HPTLC) method was established for determining echioidin in Andrographis echioides (L.) Nees (AE) plant. Echioidin isolated and identified from AE whole plant in the laboratory was used to develop the proposed HPTLC method. The method was validated in terms of linearity, limits of detection and quantification, precision, accuracy and robustness parameters. Aluminum-backed HPTLC plates precoated with silica gel 60F₂₅₄ were used as the stationary phase and chloroform-methanol (85:15, V/V) as the mobile phase. Densitometric scanning was performed using CAMAG TLC Scanner 3 at 266 nm with deuterium lamp. The linear regression analysis data for the calibration plots of echioidin showed good linearity relationship with r² = 0.9996, in the concentration range of 200–1000 ng spot^‒1. The mean ± SD values of the slope and intercept were 7.7662 ± 0.079, 218.76 ± 26.62. The stability study suggested that echioidin solution in methanol was stable within 24 h at room temperature. The average percentage recoveries of echioidin from the methanolic extracts of AE aerial parts and roots were 98.18 ± 0.28 and 98.99 ± 0.93, respectively, with % w/w content 6.07 ± 0.11 and 4.49 ± 0.07 on dry weight basis. The limits of detection and quantification were found to be 11.31 and 34.27 ng, respectively.

建立了一种简单、可靠且可重复的高效薄层色谱 (HPTLC) 方法，用于测定穿心莲(L.) Nees (AE) 植物中的海参素。在实验室中从 AE 整株植物中分离和鉴定的海胆素用于开发所提出的 HPTLC 方法。该方法在线性、检测限和定量限、精密度、准确度和稳健性参数方面得到验证。以预涂硅胶 60F _{254 的}铝背 HPTLC 板为固定相，氯仿-甲醇（85:15，V / V) 作为流动相。使用 CAMAG TLC Scanner 3 在 266 nm 和氘灯下进行光密度扫描。 在 200–1000 ng spot ^‒1浓度范围内，海参皂苷校准图的线性回归分析数据显示出良好的线性关系，r ² = 0.9996 。斜率和截距的平均值 ± SD 值为 7.7662 ± 0.079、218.76 ± 26.62。稳定性研究表明，海参皂苷的甲醇溶液在室温下 24 小时内是稳定的。从 AE 地上部分和根的甲醇提取物中提取的海参皂苷的平均回收率分别为 98.18 ± 0.28 和 98.99 ± 0.93，% w / w干重的含量分别为 6.07 ± 0.11 和 4.49 ± 0.07。发现检测限和定量限分别为 11.31 和 34.27 ng。