Calabrian licorice (roots of G. glabra var. typica), PDO certified, is one of the most valuable commercial within Glycyrrhiza species. Specialized metabolites profiling of below and aboveground parts of PDO Calabrian G. glabra was established by untargeted UHPLC-HRMS either to elucidate their distinctive accumulation among tissues and to gain insight about the chemical composition of leaves and exudate, also to provide fingerprints for PDO products authentication. Roots showed the typical profile of G. glabra, namely glabridin and prenylated phenolic compounds (erybacin B, kanzonol Y, mulberrofuran K isomers, 3-hydroxyglabrol and glabrol) were confirmed as useful species-specific markers for authenticity and traceability of Calabrian licorice. Aerial tissues contained exclusively phenolics compounds, with pinocembrin, prenylated flavanones (licoflavanone and glabranin) and dihydrostilbenes as distinctive metabolites accumulated in the leaf-exudates, and glycosylated flavonoids (isoquercitrin, astragalin and their malonyl derivatives) distributed solely in the inner leaf tissues and flowers. This comparative study provides insights on tissue-distribution of G. glabra specialized metabolites and paves the way for sustainable valorization of untapped resources and improves the economic value of supply licorice-chain.

卡拉布里亚甘草（G. glabra var. typica 的根）经过 PDO 认证，是甘草物种中最有价值的商品之一。PDO Calabrian G. glabra地下和地上部分的专门代谢物分析是通过非靶向 UHPLC-HRMS 建立的，目的是阐明它们在组织中的独特积累并深入了解叶子和渗出液的化学成分，也为 PDO 产品认证提供指纹. 根显示出G. glabra的典型轮廓，即光甘草定和异戊二烯化酚类化合物（erybacin B、kanzonol Y、mulberrofuran K 异构体、3-羟基光甘草醇和光甘草醇）被确认为有用的物种特异性标记，用于卡拉布里亚甘草的真实性和可追溯性。气生组织仅含有酚类化合物，松香素、异戊二烯化黄烷酮（licoflavanone 和 glabranin）和二氢芪作为独特代谢物积累在叶分泌物中，糖基化黄酮类化合物（异槲皮苷、黄芪甲苷及其丙二酰衍生物）仅分布在叶内部组织和花中. 这项比较研究提供了关于G. glabra特殊代谢物的组织分布的见解，并为未开发资源的可持续增值铺平了道路，并提高了供应甘草链的经济价值。