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Marinobacterium alkalitolerans sp. nov., with nitrate reductase and urease activity isolated from green algal mat collected from a solar saltern
Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2021-06-01 , DOI: 10.1007/s10482-021-01582-y
Anil Kumar Pinnaka 1, 2 , Naga Radha Srinivas Tanuku 2, 3 , Vasundhara Gupta 1 , Gunjan Vasudeva 1 , Sudharani Pydi 3 , Nishant Kashyap 1 , Swarnaprava Behera 2, 3 , Sampath Kumar Ganta 3
Affiliation  

A novel Gram-staining-negative, rod-shaped, 0.6–0.8 µm wide and 2.0–3.0 µm in length, motile bacterium designated strain AK62T, was isolated from the green algal mat collected from saltpan, Kakinada, Andhra Pradesh, India. Colonies on ZMA were circular, off-white, shiny, moist, translucent, 1–2 mm in diameter, flat, with an entire margin. The major fatty acids include C16:0, C18:1 ω7c, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c and/or iso-C14:0 3-OH). Polar lipids include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified phospholipids, and one unidentified lipid. Polyamine includes Spermidine. The DNA G + C content of the strain AK62T was 58.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain AK62T was closely related to the type strains Marinobacterium sediminicola, Marinobacterium coralli and Marinobacterium stanieri with a pair-wise sequence similarity of 96.9, 96.6 and 96.6%, respectively, forming a distinct branch within the genus Marinobacterium and clustered with M. stanieri, M. sediminicola, M. coralli and M. maritimum cluster. Strain AK62T shares average nucleotide identity (ANIb, based on BLAST) of 78.44, 76.69, and 76.95% with M. sediminicola CGMCC 1.7287T, M. stanieri DSM 7027T, and Marinobacterium halophilum Mano11T respectively. Based on the observed phenotypic, chemotaxonomic characteristics, and phylogenetic analysis, strain AK62T is described in this study as a novel species in the genus Marinobacterium, for which the name Marinobacterium alkalitolerans sp. nov. is proposed. The type strain of M. alkalitolerans is AK62T (= MTCC 12102T = JCM 31159T = KCTC 52667T).



中文翻译:

耐碱海杆菌属 11 月,具有从日光盐场收集的绿藻垫中分离出的硝酸盐还原酶和脲酶活性

从印度安得拉邦卡基纳达盐田收集的绿藻垫中分离出一种新的革兰氏染色阴性、杆状、宽 0.6–0.8 µm、长 2.0–3.0 µm 的活动细菌,命名为 AK62 T菌株。ZMA 上的菌落呈圆形,灰白色,有光泽,湿润,半透明,直径 1-2 毫米,扁平,有整个边缘。主要脂肪酸包括 C 16:0、C 18:1 ω 7 c和总和特征 3(C 16:1 ω7c和/或 C 16:1 ω6c和/或 iso-C 14:03-OH)。极性脂质包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、三种未鉴定的磷脂和一种未鉴定的脂质。多胺包括亚精胺。AK62 T菌株的DNA G + C含量为58.8 mol%。基于16S rRNA基因序列的系统发育分析表明,菌株AK62 T与典型菌株Marinobacterium sediminicolaMarinobacteriumcoralliMarinobacterium stanieri密切相关,成对序列相似性分别为96.9%、96.6%和96.6%,形成了一个明显的分支。 Marinobacterium属并与M. stanieri聚集在一起,M. sediminicolaM.coralliM. maritimum簇。菌株 AK62 T与M. sediminicola CGMCC 1.7287 TM. stanieri DSM 7027 T和Marinobacterium halophilum Mano11 T的平均核苷酸同一性(ANIb,基于 BLAST)分别为 78.44、76.69 和 76.95% 。根据观察到的表型、化学分类学特征和系统发育分析,本研究中将菌株 AK62 T描述为海杆菌属中的一个新物种,其名称为耐碱海杆菌sp。十一月 被提议。耐碱分枝杆菌的典型菌株是 AK62 T ( = MTCC 12102 T  = JCM 31159 T  = KCTC 52667 T )。

更新日期:2021-06-01
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