Adhesion molecules play an important role in urinary calculus formation. The expressions of adhesion molecules in renal tubular has been reported in some animals. However, the role of adhesion molecules in the process of sheep urinary calculus formation is still unclear. The magnesium ammonium phosphate (MAP) is the main component of sheep urinary calculus. In this paper, the sheep renal tubular epithelial cells (RTECs) were isolated and treated with MAP, the expressions of osteopontin (OPN), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and apoptosis-related indicators caspase-3, Bcl-2 and Bax in RTECs were observed, the viability of RTECs was detected by Cell Counting Kit-8 (CCK-8).

The levels of superoxide dismutase (SOD) and malondialdehyde (MDA), and the expressions of inflammatory factors Interleukin-6 (IL-6), Interleukin-1 (IL-1), Interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent (ELISA). The histopathological observation of kidney in urolithiasis sheep was made. The results showed that MAP could reduce the viability and SOD activity, enhance the activity of MDA significantly and promote the expressions of IL-1, IL-6, IL-17 and TNF-α of RTECs. By western blot and qPCR methods, the expressions of ICAM-1, VCAM-1 and OPN increased in 48 h. In addition, the expression of caspase-3 increased significantly and the ratio of Bcl-2/Bax reduced with exposure to MAP. The renal tissue structure was seriously damaged, the RTECs in urolithiasis sheep were degenerative and necrotic.

粘附分子在尿路结石形成中起重要作用。在一些动物中已经报道了肾小管中粘附分子的表达。然而，黏附分子在羊尿路结石形成过程中的作用尚不清楚。磷酸铵镁（MAP）是羊尿路结石的主要成分。本文对羊肾小管上皮细胞（RTECs）进行分离和MAP处理，观察骨桥蛋白（OPN）、细胞间粘附分子-1（ICAM-1）、血管细胞粘附分子-1（VCAM-1）的表达观察RTECs细胞凋亡相关指标caspase-3、Bcl-2、Bax，Cell Counting Kit-8（CCK-8）检测RTECs的活力。

超氧化物歧化酶（SOD）和丙二醛（MDA）水平，炎症因子白介素6（IL-6）、白介素1（IL-1）、白介素17（IL-17）和肿瘤坏死因子的表达-α (TNF-α) 通过酶联免疫吸附 (ELISA) 进行测量。对尿石症羊肾脏进行组织病理学观察。结果表明，MAP可降低细胞活力和SOD活性，显着提高MDA的活性，促进RTECs的IL-1、IL-6、IL-17和TNF-α的表达。通过蛋白质印迹和qPCR方法，ICAM-1、VCAM-1和OPN的表达在48小时内增加。此外，随着暴露于MAP，caspase-3的表达显着增加，Bcl-2/Bax的比率降低。肾组织结构严重受损，尿石症羊RTECs退行性坏死。