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Light spectrum modifies the drought-induced changes of glutathione and free amino acid levels in wheat
Acta Physiologiae Plantarum ( IF 2.4 ) Pub Date : 2021-06-01 , DOI: 10.1007/s11738-021-03253-x
M. Gyugos , M. Ahres , Z. Gulyás , G. Szalai , É. Darkó , Z. Mednyánszky , N. Dey , R. K. Kar , L. Simon-Sarkadi , G. Kocsy

The proposed spectral control of the adaptive metabolic responses to drought was tested by the comparison of two wheat genotypes with different stress tolerance. White light was used as the reference to see the effects of the three altered ratios of blue, red, and far-red spectral components, which conditions are referred to as blue, pink, and far-red lights. Under all spectral conditions, drought greatly reduced the growth of both genotypes. However, the glutathione content decreased and the proline level increased, independently of the spectrum, only in the sensitive genotype. Far-red light greatly decreased the amount of cystine and glutathione disulphide during the stress which resulted in their lower ratios compared to the reduced forms only in the tolerant genotype. The maintained more reducing redox environment contributes to its better stress tolerance. In far-red light, drought induced a greater accumulation of several free amino acids (mHis, Val, Ile, Leu, Asn, His, Tyr, Lys, Arg) in the tolerant genotype, while a smaller one in the sensitive genotype compared to the other spectral conditions. The transcript level of the genes related to amino acid and glutathione metabolism was also different between the two genotypes under this condition. The present results indicate the adaptive adjustment of glutathione and amino acid levels by far-red light during drought which observation can serve as a basis for the spectrum-dependent modification of the protective metabolites (glutathione, proline) of crops to reduce the stress-induced damages.



中文翻译:

光谱改变干旱引起的小麦谷胱甘肽和游离氨基酸水平的变化

通过比较具有不同胁迫耐受性的两种小麦基因型,测试了对干旱适应性代谢反应的拟议光谱控制。白光被用作参考,以查看蓝色、红色和远红色光谱分量的三种改变比率的效果,这些条件被称为蓝色、粉红色和远红色光。在所有光谱条件下,干旱大大降低了两种基因型的生长。然而,仅在敏感基因型中,谷胱甘肽含量降低,脯氨酸水平升高,与光谱无关。远红光在应激期间大大减少了胱氨酸和谷胱甘肽二硫化物的量,这导致它们的比率低于仅在耐受基因型中的还原形式。维持的更多还原氧化还原环境有助于其更好的耐压性。在远红光下,干旱在耐受基因型中诱导了几种游离氨基酸(mHis、Val、Ile、Leu、Asn、His、Tyr、Lys、Arg)的更多积累,而敏感基因型中的游离氨基酸较小其他光谱条件。在这种情况下,两种基因型之间与氨基酸和谷胱甘肽代谢相关的基因的转录水平也不同。目前的结果表明,干旱期间远红光对谷胱甘肽和氨基酸水平的适应性调节,该观察可作为对作物保护性代谢物(谷胱甘肽、脯氨酸)进行光谱依赖性修饰以减少胁迫诱导的基础。损害。干旱诱导耐受基因型中几种游离氨基酸(mHis、Val、Ile、Leu、Asn、His、Tyr、Lys、Arg)的更多积累,而与其他光谱条件相比,敏感基因型中的游离氨基酸较小。在这种情况下,两种基因型之间与氨基酸和谷胱甘肽代谢相关的基因的转录水平也不同。目前的结果表明,干旱期间远红光对谷胱甘肽和氨基酸水平的适应性调节,该观察可作为对作物保护性代谢物(谷胱甘肽、脯氨酸)进行光谱依赖性修饰以减少胁迫诱导的基础。损害。干旱诱导耐受基因型中几种游离氨基酸(mHis、Val、Ile、Leu、Asn、His、Tyr、Lys、Arg)的更多积累,而与其他光谱条件相比,敏感基因型中的游离氨基酸较小。在这种情况下,两种基因型之间与氨基酸和谷胱甘肽代谢相关的基因的转录水平也不同。目前的结果表明,干旱期间远红光对谷胱甘肽和氨基酸水平的适应性调节,该观察可作为对作物保护性代谢物(谷胱甘肽、脯氨酸)进行光谱依赖性修饰以减少胁迫诱导的基础。损害。而与其他光谱条件相比,敏感基因型中的较小。在这种情况下,两种基因型之间与氨基酸和谷胱甘肽代谢相关的基因的转录水平也不同。目前的结果表明,干旱期间远红光对谷胱甘肽和氨基酸水平的适应性调节,该观察可作为对作物保护性代谢物(谷胱甘肽、脯氨酸)进行光谱依赖性修饰以减少胁迫诱导的基础。损害。而与其他光谱条件相比,敏感基因型中的较小。在这种情况下,两种基因型之间与氨基酸和谷胱甘肽代谢相关的基因的转录水平也不同。目前的结果表明,干旱期间远红光对谷胱甘肽和氨基酸水平的适应性调节,该观察可作为对作物保护性代谢物(谷胱甘肽、脯氨酸)进行光谱依赖性修饰以减少胁迫诱导的基础。损害。

更新日期:2021-06-01
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