Burdock root is the root of Arctium lappa L., a plant of the Compositae family, which has the effects of dispersing wind and heat, detoxifying and reducing swelling. In order to better control the quality of burdock root, a screening study of quality control indicators was carried out. The current research combines biological activity evaluation with chemical analysis to screen and identify the biologically active compounds of burdock root as chemical components for the quality control of herbal medicine. The efficacy of 10 batches of ethanol extracts of burdock roots was evaluated by a tumor inhibition experiment in S180 tumor-bearing mice. The five main chemical components of these extracts were simultaneously quantitatively measured by ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometry. Pearson correlation analysis was used to establish the relationship between these extracts’ biological activity and chemical properties. The results showed that chlorogenic acid, caffeic acid and cynarin were positively correlated with the effect of inhibiting tumor growth, and further bioassays confirmed this conclusion. In conclusion, chlorogenic acid, caffeic acid and cynarin can be used as quality control markers for burdock root’s antitumor effect.

中文翻译：

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结合抗肿瘤作用和UHPLC-QqQ-MS/MS发现牛蒡根中的质控成分

牛蒡根是牛蒡的根L.，菊科植物，具有散风清热、解毒消肿的功效。为了更好地控制牛蒡根的质量，开展了质量控制指标的筛选研究。目前的研究将生物活性评价与化学分析相结合，筛选和鉴定牛蒡根的生物活性化合物作为草药质量控制的化学成分。通过S180荷瘤小鼠的抑瘤实验评价了10批牛蒡乙醇提取物的疗效。采用超高效液相色谱结合三重四极杆质谱对这些提取物的五种主要化学成分同时进行定量测定。Pearson相关分析用于建立这些提取物的生物活性和化学性质之间的关系。结果表明，绿原酸、咖啡酸和洋蓟素与抑制肿瘤生长的作用呈正相关，进一步的生物测定证实了这一结论。综上所述，绿原酸、咖啡酸和洋蓟素可作为牛蒡抗肿瘤作用的质控标志物。