This study aimed at identifying genomic regions and genes associated with intramuscular fat content (IMF) in Duroc pigs using a weighted single-step GWAS. Data from 3912 pigs, of which 3770 animals were genotyped with GeneSeek Porcine 50K Bead chip, were used for the association analysis. We identified 19 genomic regions that each explained >1% of the additive genetic variance associated with IMF. Notably, a consistent QTL on SSC7 (117.42–117.92 Mb) was confirmed, explaining 3.70% of the additive genetic variance, and two genes, BDKRB2 and ATG2B, were highlighted as promising candidates for IMF. Two QTL (SSC7, 94.19–94.64 Mb; SSC14, 123.25–123.75 Mb), which harbored MED6 and MAP3K9 genes and TCF7L2 gene respectively, were newly identified as associated with IMF. In conclusion, we identified a consistent QTL and additional genomic regions and genes that contributed to the genetic variance of IMF using a large-scale sample size of genotyped pigs and genealogical information.

中文翻译：

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大规模全基因组关联分析揭示了杜洛克猪肌肉内脂肪含量的 QTL 和候选基因

本研究旨在使用加权单步 GWAS 确定与杜洛克猪肌肉内脂肪含量 (IMF) 相关的基因组区域和基因。来自 3912 头猪的数据用于关联分析，其中 3770 头动物使用 GeneSeek Porcine 50K Bead 芯片进行基因分型。我们确定了 19 个基因组区域，每个区域都解释了与 IMF 相关的加性遗传变异的 1% 以上。值得注意的是，SSC7 (117.42–117.92 Mb) 上的一致 QTL 得到证实，解释了 3.70% 的加性遗传变异，并且两个基因BDKRB2和ATG2B被强调为有希望的 IMF 候选基因。两个 QTL（SSC7，94.19–94.64 Mb；SSC14，123.25–123.75 Mb），其中包含 MED6和MAP3K9基因以及TCF7L2基因分别被新鉴定为与 IMF 相关。总之，我们使用基因分型猪的大规模样本量和家谱信息确定了一致的 QTL 和额外的基因组区域和基因，这些区域和基因有助于 IMF 的遗传变异。