Citrate-coated iron oxide nanoparticles (IONPs) have potential use in environmental remediation, with possibilities in decontaminating aquatic environments exposed to toxic substances. This study analyzed IONPs associated to Roundup Original, a glyphosate-based herbicide (GBH), and pure glyphosate (GLY), through ultrastructural and histopathological biomarkers in liver tissue, from females of Poecilia reticulata exposed to: iron ions (0.3 mg/L) (IFe) and IONPs (0.3 mgFe/L) associated with GLY (0.65 mg/L) and GBH (0.65 mgGLY/L (IONP + GBH1) and 1.30 mgGLY/L (IONP + GBH2)) for a period of 7, 14 and 21 days, followed by an equal post-exposure period only in reconstituted water. For the assays, the synthetized IONPs had crystalline and rounded shape with an average diameter of 2,90 nm, hydrodynamic diameter 66,6 mV, zeta potential −55,4 and diffraction profile of maghemite (γ-Fe₂O₃). The data obtained by biomarkers indicated a high inflammatory response in all treatments. These same parameters, considered during the post-exposure period indicated recovery in reaction patterns of circulatory disturbances and regressive changes, resulting in average reductions of 37,53 points in IFe, 21 points in IONP + GBH1, 15 points in IONP + GBH2 and 11 points in IONP + GLY in total histopathological index of liver after 21 days post-exposure. However, although the cellular and tissue responses were significant, there was no change in the condition factor and hepatosomatic index, denoting resilience of the experimental model.

柠檬酸盐涂覆的氧化铁纳米颗粒（IONP）在环境修复方面具有潜在用途，可以净化暴露于有毒物质的水生环境。本研究通过肝组织中的超微结构和组织病理学生物标志物分析了与农达原味（一种草甘膦除草剂 (GBH) 和纯草甘膦 (GLY)）相关的 IONP，这些生物标志物来自暴露于以下物质的Poecilia reticulata雌性： 铁离子 (0.3 mg/L) (IFe) 和 IONP (0.3 mgFe/L) 与 GLY (0.65 mg/L) 和 GBH (0.65 mgGLY/L (IONP + GBH1) 和 1.30 mgGLY/L (IONP + GBH2)) 相关，持续时间为 7, 14和 21 天，然后是同样的仅在复原水中的暴露后时间。对于测定，合成的IONP具有结晶和圆形形状，平均直径为2,90 nm，流体动力学直径66,6 mV，zeta电位-55,4和磁赤铁矿（γ-Fe ₂ O ₃ ）的衍射图。生物标志物获得的数据表明所有治疗均出现高炎症反应。在暴露后期间考虑的这些相同参数表明循环障碍和回归变化的反应模式恢复，导致 IFe 平均降低 37.53 点，IONP + GBH1 平均降低 21 点，IONP + GBH2 平均降低 15 点，IONP + GBH2 平均降低 11 点。暴露后21天后肝脏总组织病理学指数中IONP+GLY的分数。然而，尽管细胞和组织反应显着，但条件因子和肝体指数没有变化，表明实验模型具有弹性。