Considering that the use of psychoactive substances (PSs) is a risk factor to either higher intensity or frequency of suicidal behavior, hair analysis was conducted to investigate the most consumed PSs (opiates, amphetamine stimulants, marijuana, cocaine and heroin) in patients who attempted suicide and received urgent care at emergency service. Hair samples were extracted using methanol and sonicated under heating and then analyzed using liquid chromatography-tandem mass spectrometry. During validation, the method complied with international recommended criteria, with limits of detection between 0.0025 and 0.05 ng/mg and linearity between 0.1 and 4 ng/mg for methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), morphine, amphetamine, 6-acetylmorphine, 3,4-methylenedioxyamphetamine (MDA), fenproporex, diethylpropion and codeine; between 0.025 and 1 ng/mg for tetrahydrocannabinol (THC), benzoylecgonine and cocaethylene and between 0.25 and 10 ng/mg for cocaine and mazindol. A total of 109 hair samples were analyzed and segmented in 404 parts. Among all analyzed samples, 30.3% were positive for at least one PS (n = 33), such as cocaine (90.9%), codeine (12.1%), morphine (3.0%), MDMA (3.0%) and THC (3.0%). In segmental analysis of cocaine positive samples (n = 30), 76.7% of the samples indicated recent exposure to cocaine (<1 month). This same behavior was observed when analyzing codeine (n = 4) and morphine (n = 1). THC positive samples indicated exposure dated ∼4 months prior. In conclusion, the method was validated following international recommendations for the 12 most consumed PSs in Brazil, as well as two of the most common found metabolites.

中文翻译：

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LC-MS-MS 测定头发中滥用药物：在自杀未遂调查中的应用

考虑到精神活性物质 (PSs) 的使用是自杀行为强度或频率较高的风险因素，因此进行了头发分析以调查那些尝试过的患者中消耗最多的 PSs（鸦片制剂、苯丙胺兴奋剂、大麻、可卡因和海洛因）。自杀并在紧急服务部门接受紧急护理。使用甲醇提取头发样品并在加热下进行超声处理，然后使用液相色谱-串联质谱法进行分析。在验证过程中，该方法符合国际推荐标准，甲基苯丙胺、3,4-亚甲基二氧基甲基苯丙胺 (MDMA)、吗啡、苯丙胺、6-乙酰吗啡的检测限在 0.0025 和 0.05 ng/mg 之间，线性在 0.1 和 4 ng/mg 之间, 3,4-亚甲二氧苯丙胺 (MDA), fenproporex, diethylpropion 和可待因；0 之间。四氢大麻酚 (THC)、苯甲酰芽子碱和可卡乙烯为 025 至 1 ng/mg，可卡因和马吲哚为 0.25 至 10 ng/mg。对总共 109 个头发样本进行了分析和分割，分为 404 个部分。在所有分析的样本中，30.3% 的至少一种 PS（n = 33）呈阳性，例如可卡因（90.9%）、可待因（12.1%）、吗啡（3.0%）、摇头丸（3.0%）和四氢大麻酚（3.0%） ）。在可卡因阳性样本（n = 30）的分段分析中，76.7% 的样本表明最近接触过可卡因（<1 个月）。在分析可待因 (n = 4) 和吗啡 (n = 1) 时观察到相同的行为。THC 阳性样本表明暴露日期约为 4 个月前。总之，该方法已根据国际建议对巴西 12 种消耗最多的 PS 以及两种最常见的代谢物进行了验证。