Transplantation of bone marrow stem cells (BMSCs) has shown to have a vital role in promoting nerve regeneration after SCI. The aim of this study was to investigate the effect of BMSCs transplantation in healing of spinal cord injury (SCI) in mice based on morphologic parameters.

Forty two male mice were randomly divided into 3 groups of control with no intervention, experimental SCI without treatment, and experimental SCI transplanted with 2 × 10⁵ BMSCs intravenously. To induce SCI bilaterally, T10 was compressed for 2 min. The animals were sacrificed 3 and 5 weeks after SCI and T7-T11 segments of spinal cord were removed and stained by Giemsa and H&E methods. Stereological assessment estimated the gray and white matter volume, the number of neurons and neuroglia and diameter of central canal. The average amount of gray matter in SCI injury group was significantly lower than control group. An increase in the number of neurons was noted after cell transplantation. The number of neurons in SCI injury group significantly decreased in comparison to the control group. In cell transplantation group, a significant increase in the number of neurons was visible when compared to SCI injury group. The increase in the number of neurons after cell transplantation denotes to the regenerative potential of BMSCs in SCI. These findings can be added to the literature and open a new window when targeting treatment of SCI.

骨髓干细胞 (BMSCs) 的移植在促进 SCI 后的神经再生方面具有重要作用。本研究的目的是根据形态学参数研究骨髓间充质干细胞移植对小鼠脊髓损伤 (SCI) 愈合的影响。

42只雄性小鼠随机分为3组，对照组不干预，实验性SCI不治疗，实验性SCI移植2×10 ⁵静脉注射骨髓间充质干细胞。为了双侧诱发 SCI，T10 被压缩 2 分钟。SCI 后 3 周和 5 周处死动物，取出脊髓 T7-T11 节段并通过 Giemsa 和 H&E 方法染色。体视评估估计灰质和白质体积、神经元和神经胶质细胞的数量以及中央管的直径。SCI损伤组灰质平均量显着低于对照组。在细胞移植后注意到神经元数量的增加。与对照组相比，SCI损伤组的神经元数量显着减少。在细胞移植组中，与SCI损伤组相比，神经元数量明显增加。