ABSTRACT

Extracellular vesicles (EVs) are specific subcellular vesicles released by cells under various environmental conditions. Tumescent liposuction is a commonly used procedure in plastic surgery practice. In the present study, we aimed to extract EVs derived from lipoaspirate fluid (LF-EVs) and characterize them using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The global profiles of proteins and microRNAs from LF-EVs were identified, strongly suggesting a potential regulatory function of LF-EVs. In addition, we investigated the effects and mechanisms of LF-EVs on fat graft survival. Cell functional tests showed that LF-EVs promoted the proliferation, migration, and tube structure formation of human umbilical vein endothelial cells. LF-EVs also promoted the adipogenic differentiation of adipose tissue-derived stem cells. The results of animal experiments showed that the average weights of fat grafts in the LF-EVs-treated group were significantly higher than those in the control group. Histologically, there was less fibrosis, fewer cysts, and increased fat tissue survival in the LF-EVs group. Further investigations of angiogenic and adipogenic factors revealed that LF-EVs also promoted angiogenesis and exerted a pro-adipogenic effect in vivo. Our findings will help to elucidate the functions of LF-EVs and provide a reference dataset for future translational studies.

摘要

细胞外囊泡 (EVs) 是细胞在各种环境条件下释放的特定亚细胞囊泡。肿胀吸脂术是整形外科实践中常用的手术。在本研究中，我们旨在提取来自脂肪抽吸液 (LF-EV) 的 EV，并使用透射电子显微镜、纳米粒子跟踪分析和蛋白质印迹对其进行表征。鉴定了来自 LF-EVs 的蛋白质和 microRNAs 的全球概况，强烈表明 LF-EVs 的潜在调节功能。此外，我们研究了 LF-EVs 对脂肪移植存活率的影响和机制。细胞功能测试表明LF-EVs促进了人脐静脉内皮细胞的增殖、迁移和管状结构的形成。LF-EVs 还促进了脂肪组织来源的干细胞的成脂分化。动物实验结果表明，LF-EVs治疗组脂肪移植物的平均重量显着高于对照组。在组织学上，LF-EVs 组的纤维化较少，囊肿较少，脂肪组织存活率增加。对血管生成和脂肪生成因子的进一步研究表明，LF-EVs 还促进血管生成并发挥促脂肪生成作用体内。我们的发现将有助于阐明 LF-EV 的功能，并为未来的转化研究提供参考数据集。