ZrO₂ nano-porous films with different morphologies were prepared by anodic oxidation method, and then rhodamine B (RB) and fluorescein (FL) were immobilized on the surface of ZrO₂ to obtain composite fluorescent films. The effects of ZrO₂ morphology, loading amount of RB and FL, excitation wavelength, and FL/RB ratio on the fluorescence performance of the composite films and the FL-RB fluorescence resonance energy transfer (FRET) system were studied in detail. The related reasons were investigated as well. Results show that the ZrO₂ film with rough surface and tiny pores is more favorable for fluorescence emission. In the FRET system, the energy transfer efficiency of FL and the actual enhancement effect on RB vary with the FL/RB ratio and the loading amount. Moreover, the fluorescence enhancement effect of FL on RB is also closely related to the excitation wavelength. Compared with that in aqueous solutions, immobilization on the optimized ZrO₂ nano-porous film can dramatically improve the fluorescence performance of RB and FL, making their emission intensity increased by 4.5 and 5.7 times, respectively. The efficiency of FRET system on ZrO₂ nano-porous film can reach more than 73.2%, which is significantly higher than that in aqueous solution (23.6%).

采用阳极氧化法制备了不同形貌的ZrO ₂纳米多孔薄膜，然后将罗丹明B（RB）和荧光素（FL）固定在ZrO ₂表面，得到复合荧光薄膜。详细研究了ZrO ₂形貌、RB和FL的负载量、激发波长和FL/RB比对复合薄膜和FL-RB荧光共振能量转移(FRET)系统荧光性能的影响。相关原因也进行了调查。结果表明，ZrO ₂表面粗糙、微孔的薄膜更有利于荧光发射。在 FRET 系统中，FL 的能量传递效率和对 RB 的实际增强效果随 FL/RB 比和加载量而变化。此外，FL对RB的荧光增强效果也与激发波长密切相关。与水溶液相比，固定在优化的ZrO ₂纳米多孔膜上可以显着提高RB和FL的荧光性能，使其发射强度分别提高4.5倍和5.7倍。FRET系统对ZrO ₂纳米多孔膜的效率可达73.2%以上，明显高于水溶液（23.6%）。