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Encapsulation of purple sweet potato's anthocyanin in CMC-PVA matrix for development of paper strips as a colorimetric biosensor
Indian Journal of Biochemistry and Biophysics ( IF 1.5 ) Pub Date : 2021-06-01
Anting Wulandari, Titi Candra Sunarti, Farah Fahma, Erliza Noor, Toshiharu Enomae

Bioactive compound from local resources such as anthocyanin has a high potential to develop as a biosensor for nitrite detection. As a biosensor, this compound shows instability to certain environmental conditions. To overcome this, bioactive compound may need to encapsulate. The aim of this work is to encapsulate of anthocyanin in the CMC-PVA matrix to improve its stability as paper strip for sodium nitrite detection. The result of this work shows that anthocyanin encapsulated by CMC/PVA matrix has well formed. Encapsulated anthocyanin has good homogeneity and it was strongly immobilized in the CMC/PVA matrix. Encapsulated anthocyanin is attached to the surface of the paper medium and penetrated into the matrix. The strip is shown changing color from red to yellow as it applied to nitrite. The selectivity of biosensor paper strip system is observed for various other chemicals including heavy metals such as Pb2+, Zn2+, Fe3+, Mg2+, NaCl, boric acid, monosodium glutamate, sodium benzoate, and formalin with a concentration of 10000 ppm, respectively, and the biosensor was selective towards nitrite. This work gives a basic information to development of a practical green sensor in the form of paper strips for nitrite detection with good stability and selectivity.

中文翻译:

紫甘薯花青素在 CMC-PVA 基质中的封装,用于开发纸条作为比色生物传感器

来自当地资源的生物活性化合物,如花青素,作为亚硝酸盐检测的生物传感器具有很高的开发潜力。作为生物传感器,这种化合物对某些环境条件表现出不稳定性。为了克服这一点,生物活性化合物可能需要封装。这项工作的目的是将花青素封装在 CMC-PVA 基质中,以提高其作为亚硝酸钠检测纸条的稳定性。这项工作的结果表明,CMC/PVA 基质包裹的花青素已形成良好。包封的花青素具有良好的均一性,并且牢固地固定在 CMC/PVA 基质中。封装的花青素附着在纸介质的表面并渗透到基质中。显示条带在应用于亚硝酸盐时颜色从红色变为黄色。2+、Zn 2+、Fe 3+、Mg 2+、NaCl、硼酸、谷氨酸钠、苯甲酸钠和福尔马林浓度分别为10000 ppm,生物传感器对亚硝酸盐具有选择性。这项工作为开发具有良好稳定性和选择性的用于亚硝酸盐检测的纸条形式的实用绿色传感器提供了基本信息。
更新日期:2021-06-01
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