This study intended to investigate the effects of altitudinal gradients on the accumulation of phenolic compounds and bioactivity among different populations of Lobelia nicotianifolia Roth. from Northern Western Ghats of India. High-performance liquid chromatography (HPLC) revealed a maximum content of embelin (16.36 µg/g DW), gallic acid (53.47 µg/g DW), and quercetin (18.93 µg/g DW) in Kas population (1148 m m.s.l.). L. nicotianifolia from Kas region has higher radical scavenging activity in 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays and also exhibited higher cytotoxicity against two breast cancer cell lines (MCF-7 and MDA-MB231). A correlation analysis indicates a significant relationship between altitudinal gradients of L. nicotianifolia population and accumulation of phenolic compounds and bioactivity. Principle component analysis (PCA) and hierarchical cluster analysis (HCA) grouped L. nicotianifolia populations into different clusters based on their altitudes. L. nicotianifolia from Kas region (1148 m) is selected as an elite population because of its potential to accumulate higher phenolic compounds and subsequent bioactivity.

中文翻译：

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海拔梯度影响药学上重要的酚类化合物在 Lobelia nicotianifolia Roth 的叶子中的积累。并调节其抗氧化和抗癌特性

本研究旨在研究海拔梯度对Lobelia nicotianifolia Roth不同种群酚类化合物积累和生物活性的影响。来自印度西北部的高止山脉。高效液相色谱 (HPLC) 显示 Kas 种群 (1148 m msl) 中的 embelin (16.36 µg/g DW)、没食子酸 (53.47 µg/g DW) 和槲皮素 (18.93 µg/g DW) 含量最高。烟草来自 Kas 区域的 2,2-二苯基-1-苦基肼 (DPPH) 和 2,2-叠氮基-双(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 检测中具有更高的自由基清除活性，并且对两种乳腺癌细胞系（MCF-7 和 MDA-MB231）。相关性分析表明L 的海拔梯度之间存在显着关系。nicotianifolia种群和酚类化合物的积累和生物活性。主成分分析 (PCA) 和层次聚类分析 (HCA) 将L分组。nicotianifolia种群根据海拔高度分为不同的集群。烟草 Kas 地区 (1148 m) 被选为精英种群，因为它有可能积累更高的酚类化合物和随后的生物活性。