The pluripotency transcription factor OCT4 represses heme oxygenase-1 gene expression,FEBS Letters

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The pluripotency transcription factor OCT4 represses heme oxygenase-1 gene expression
FEBS Letters ( IF 3.0 ) Pub Date : 2021-05-31 , DOI: 10.1002/1873-3468.14138
María Victoria Petrone ₁ , Ayelén Toro ₁ , Camila Vazquez Echegaray ₁ , Marcos Gabriel Francia ₁ , Claudia Solari ₁ , Maria Soledad Cosentino ₁ , Elba Vazquez _{2,

3} , Alejandra Guberman _{3,

4}

Affiliation

In embryonic stem (ES) cells, oxidative stress control is crucial for genomic stability, self-renewal, and cell differentiation. Heme oxygenase-1 (HO-1) is a key player of the antioxidant system and is also involved in stem cell differentiation and pluripotency acquisition. We found that the HO-1 gene is expressed in ES cells and induced after promoting differentiation. Moreover, downregulation of the pluripotency transcription factor (TF) OCT4 increased HO-1 mRNA levels in ES cells, and analysis of ChIP-seq public data revealed that this TF binds to the HO-1 gene locus in pluripotent cells. Finally, ectopic expression of OCT4 in heterologous systems repressed a reporter carrying the HO-1 gene promoter and the endogenous gene. Hence, this work highlights the connection between pluripotency and redox homeostasis.

中文翻译：

多能性转录因子 OCT4 抑制血红素加氧酶-1 基因表达

在胚胎干 (ES) 细胞中，氧化应激控制对于基因组稳定性、自我更新和细胞分化至关重要。血红素加氧酶-1 (HO-1) 是抗氧化系统的关键参与者，也参与干细胞分化和多能性获得。我们发现 HO-1 基因在 ES 细胞中表达并在促进分化后被诱导。此外，多能性转录因子 (TF) OCT4 的下调增加了 ES 细胞中的 HO-1 mRNA 水平，对 ChIP-seq 公开数据的分析表明，该 TF 与多能细胞中的 HO-1 基因位点结合。最后，OCT4 在异源系统中的异位表达抑制了携带 HO-1 基因启动子和内源基因的报告基因。因此，这项工作强调了多能性和氧化还原稳态之间的联系。

更新日期：2021-07-27

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