Abstract

Hyaluronic acid (HA) is a biopolymer with applications in different areas such as medicine and cosmetics. HA is currently either isolated from animal sources or produced by microbial fermentation. Animal HA presents some disadvantages such as high cost and risk of viral cross-species or another infectious agent. In the present study, we evaluated the physicochemical characteristics and in vitro antioxidant capacity of HA produced by Streptococcus zooepidemicus CCT 7546. In addition, commercial sodium hyaluronate (SH) from an animal source was used as control. The microbial HA yield after purification was 69.8 mg/L. According to Fourier transform infrared spectroscopy, it was seen that bacterial and animal HA spectra are overlapped. The thermogravimetric analysis revealed that microbial HA was more stable than its equivalent from the animal source. However, scanning electron microscopy indicates that the purification method used in the animal product was more effective. Microbial HA showed activity in total antioxidant capacity (14.02 ± 0.38%), reducing power (18.18 ± 6.43%), DPPH radical-scavenging (5.57 ± 0.23 kmol TE/g), and hydroxyl radical-scavenging (28.39 ± 2.40%) tests. Therefore, in vitro antioxidant tests demonstrated that the antioxidant action mechanism occurs through scavenging reactive oxygen species (ROS) and donating electrons/hydrogen atoms.

摘要

透明质酸 (HA) 是一种生物聚合物，可应用于医药和化妆品等不同领域。目前 HA 要么从动物来源中分离出来，要么通过微生物发酵产生。动物 HA 存在一些缺点，例如高成本和病毒跨物种或其他传染因子的风险。在本研究中，我们评估了兽疫链球菌产生的 HA的理化特性和体外抗氧化能力。CCT 7546。此外，来自动物来源的商业透明质酸钠 (SH) 用作对照。纯化后的微生物HA产量为69.8 mg/L。根据傅里叶变换红外光谱，可以看出细菌和动物 HA 光谱是重叠的。热重分析表明，微生物 HA 比动物来源的等效物更稳定。然而，扫描电子显微镜表明，在动物产品中使用的纯化方法更有效。微生物 HA 在总抗氧化能力 (14.02 ± 0.38%)、还原能力 (18.18 ± 6.43%)、DPPH 自由基清除 (5.57 ± 0.23 kmol TE/g) 和羟基自由基清除 (28.39 ± 2.40%) 测试中显示出活性. 因此，体外抗氧化测试表明，抗氧化作用机制是通过清除活性氧 (ROS) 和提供电子/氢原子来实现的。