Abstract

Flow cytometry is a useful ancillary tool for the diagnosis of nodal B cell lymphomas. Well-established antigens have diagnostic limitations. This study aimed to assess the expression of CD71, CD81, CD44 and CD39 by flow cytometry in B cell lymphomas. Expression of these 4 antigens was queried in 185 samples with a diagnosis of a B cell lymphoma according to a histological examination of the lymph node and the World Health Organization (WHO) classification (follicular lymphoma [FL, n = 96], diffuse large B cell lymphoma/High grade B cell lymphoma [DLBCL/HGBH, n = 48], marginal zone lymphoma/lymphoplasmacytic lymphoma [MZL/LPL, n = 14], chronic lymphocytic leukemia/small lymphocytic lymphoma [CLL, n = 10], mantle cell lymphoma [MCL, n = 11], Burkitt lymphoma [BL, n = 4] and other [n = 2]). CD81 was bright and CD44 was dim in germinal center-derived malignancies, particularly aggressive lymphomas (BL and CD10-positive DLBCL/HGBL). CD81 was very dim in CLL. CD71 was bright in aggressive lymphomas (DLBCL/HGBL and BL). CD39 was bright in CD10-negative DLBCL. CD71 appeared valuable in the differential diagnosis between indolent and aggressive lymphomas, CD39 between CD10-negative DLBCL and MZL/LPL and CD81 between MCL and CLL. To conclude, we report the expression of CD71, CD81, CD44 and CD39 by FC in B cell lymphomas. Further studies will have to determine the value they add to specific FC panels.

摘要

流式细胞术是诊断淋巴结 B 细胞淋巴瘤的有用辅助工具。公认的抗原具有诊断局限性。本研究旨在通过流式细胞术评估 B 细胞淋巴瘤中 CD71、CD81、CD44 和 CD39 的表达。根据淋巴结的组织学检查和世界卫生组织 (WHO) 分类（滤泡性淋巴瘤 [FL，n  = 96]，弥漫性大 B细胞淋巴瘤/高级别 B 细胞淋巴瘤 [DLBCL/HGBH，n  = 48]，边缘区淋巴瘤/淋巴浆细胞淋巴瘤 [MZL/LPL，n  = 14]，慢性淋巴细胞白血病/小淋巴细胞淋巴瘤 [CLL，n  = 10]，地幔细胞淋巴瘤 [MCL,n  = 11]、伯基特淋巴瘤 [BL, n  = 4] 和其他 [ n  = 2])。在生发中心衍生的恶性肿瘤，特别是侵袭性淋巴瘤（BL 和 CD10 阳性 DLBCL/HGBL）中，CD81 是明亮的，而 CD44 是暗淡的。CD81 在 CLL 中非常暗淡。CD71 在侵袭性淋巴瘤（DLBCL/HGBL 和 BL）中是明亮的。CD39 在 CD10 阴性 DLBCL 中是明亮的。CD71 在惰性淋巴瘤和侵袭性淋巴瘤的鉴别诊断中显得有价值，CD39 在 CD10 阴性 DLBCL 和 MZL/LPL 之间以及 CD81 在 MCL 和 CLL 之间显得有价值。总之，我们报告了 FC 在 B 细胞淋巴瘤中的 CD71、CD81、CD44 和 CD39 表达。进一步的研究将必须确定它们为特定 FC 面板增加的价值。