In this study, response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction parameters of Sojae Semen Praeparatum polysaccharides (SSPP-80), the optimum conditions were determined as follows: ultrasonic frequency of 100 W, ultrasonic power of 80 Hz, ultrasonic temperature of 52℃, ultrasonic time of 23 minutes, and liquid to raw material ratio of 40 mL/g. Based on these conditions, polysaccharides extraction rate was 7.72% ± 0.26%. Then, 2 novel polysaccharides (SSPP-80‐1, SSPP-80‐2) were isolated from SSPP by DEAE-cellulose 52 chromatography. The chemical compositions, physicochemical properties, and structure of SSPPs were investigated by simultaneous thermal analyzer (TGA), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FI-IR), and high-performance liquid chromatography (HPLC). The results showed that SSPP-80 and 2 fractions were mainly composed of mannose (Man), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara). In addition, the antioxidant activities were evaluated against the DPPH and hydroxyl radical in vitro, the IC₅₀ of SSPP-80, SSPP-80‐1 and SSPP-80‐2 against DPPH free radical were 4.407, 8.267, and 5.204 mg/mL, respectively, whereas the IC₅₀values for removing hydroxyl groups were 5.318, 3.516, and 4.016 mg/mL, respectively. It demonstrated that SSPP-80 and 2 fractions had certain antioxidant activity. Theoretical basis for use of Sojae Semen Praeparatum polysaccharides was provided by this study.

本研究采用响应面法（RSM）对大豆油多糖（SSPP-80）的超声辅助提取参数进行优化，确定最佳条件为：超声频率100 W，超声功率80 Hz ，超声温度52℃，超声时间23分钟，料液比40mL/g。基于这些条件，多糖提取率为7.72%±0.26%。然后，通过DEAE-纤维素52层析从SSPP中分离出2种新型多糖（SSPP-80-1、SSPP-80-2）。通过同时热分析仪 (TGA)、扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FI-IR) 和高效液相色谱 (HPLC) 研究了 SSPPs 的化学成分、理化性质和结构。结果表明SSPP-80和2个馏分主要由甘露糖(Man)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)和阿拉伯糖(Ara)组成。此外，还评估了体外对 DPPH 和羟基自由基的抗氧化活性，IC₅₀ SSPP-80，SSPP-80-1和SSPP-80-2对DPPH自由基的是4.407，8.267，和5.204毫克/毫升，分别，而IC ₅₀为除去羟基值分别为5.318，3.516，和4.016毫克/毫升，分别。表明SSPP-80和2级分具有一定的抗氧化活性。本研究为大豆油多糖的利用提供了理论依据。