The circadian clock is the broadly conserved, protein-based, timekeeping mechanism that synchronizes biology to the Earth’s 24-h light-dark cycle. Studies of the mechanisms of circadian timekeeping have placed great focus on the role that individual protein-protein interactions play in the creation of the timekeeping loop. However, research has shown that clock proteins most commonly act as part of large macromolecular protein complexes to facilitate circadian control over physiology. The formation of these complexes has led to the large-scale study of the proteins that comprise these complexes, termed here “circadian interactomics.” Circadian interactomic studies of the macromolecular protein complexes that comprise the circadian clock have uncovered many basic principles of circadian timekeeping as well as mechanisms of circadian control over cellular physiology. In this review, we examine the wealth of knowledge accumulated using circadian interactomics approaches to investigate the macromolecular complexes of the core circadian clock, including insights into the core mechanisms that impart circadian timing and the clock’s regulation of many physiological processes. We examine data acquired from the investigation of the macromolecular complexes centered on both the activating and repressing arm of the circadian clock and from many circadian model organisms.

生物钟是一种广泛保守的、基于蛋白质的计时机制，可将生物学与地球的 24 小时明暗循环同步。对昼夜节律计时机制的研究将重点放在了个体蛋白质-蛋白质相互作用在计时循环的创建中所起的作用。然而，研究表明，时钟蛋白最常作为大分子蛋白复合物的一部分，以促进对生理的昼夜节律控制。这些复合物的形成导致了对构成这些复合物的蛋白质的大规模研究，这里称为“昼夜节律相互作用组学”。” 对构成生物钟的大分子蛋白质复合物的昼夜相互作用组学研究揭示了许多昼夜节律计时的基本原理以及昼夜节律对细胞生理学的控制机制。在这篇综述中，我们研究了使用昼夜节律相互作用组学方法积累的大量知识，以研究核心生物钟的大分子复合物，包括对赋予昼夜节律和时钟调节许多生理过程的核心机制的见解。我们检查了从以生物钟的激活和抑制臂为中心的大分子复合物的研究以及许多昼夜模式生物中获得的数据。我们检查了使用昼夜相互作用组学方法积累的大量知识，以研究核心生物钟的大分子复合物，包括对赋予昼夜节律和时钟调节许多生理过程的核心机制的见解。我们检查了从以生物钟的激活和抑制臂为中心的大分子复合物的研究以及许多昼夜模式生物中获得的数据。我们检查了使用昼夜相互作用组学方法积累的大量知识，以研究核心生物钟的大分子复合物，包括对赋予昼夜节律和时钟调节许多生理过程的核心机制的见解。我们检查了从以生物钟的激活和抑制臂为中心的大分子复合物的研究以及许多昼夜模式生物中获得的数据。