Natural products are very attractive for development of medicine. Their structure and bioactivities are often beyond human knowledge and imagination. We have developed isolation methods for target protein-oriented natural products so as quickly to discover bioactive compounds from natural resources. This review summarizes our recent results including protein beads methods for neural stem cells differentiation activators and new cancer drug candidates. Syntheses of isolated compounds are described. We also developed protein plate method for identification of protein–protein interaction inhibitors. Because protein binding ability is tightly related to bioactivity, protein-based natural products isolation is a powerful means to find new candidate medicines.

天然产物对医学的发展非常有吸引力。它们的结构和生物活性往往超出人类的知识和想象。我们开发了以目标蛋白质为导向的天然产物的分离方法，以便快速从自然资源中发现生物活性化合物。这篇综述总结了我们最近的结果，包括用于神经干细胞分化激活剂和新的癌症候选药物的蛋白质珠方法。描述了分离化合物的合成。我们还开发了用于鉴定蛋白质-蛋白质相互作用抑制剂的蛋白质平板法。由于蛋白质结合能力与生物活性密切相关，基于蛋白质的天然产物分离是寻找新候选药物的有力手段。