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Assaying of Cu2+ with near-infrared l-cysteine-capped CdSeTe/CdS quantum dots and its effect on cell imaging
Luminescence ( IF 3.2 ) Pub Date : 2021-05-28 , DOI: 10.1002/bio.4096 Huan Zhang 1 , Zhenzhen Li 1 , Huaying Huang 1 , Si Ouyang 1 , Yiqing Deng 1 , Qiang Zhao 1
Luminescence ( IF 3.2 ) Pub Date : 2021-05-28 , DOI: 10.1002/bio.4096 Huan Zhang 1 , Zhenzhen Li 1 , Huaying Huang 1 , Si Ouyang 1 , Yiqing Deng 1 , Qiang Zhao 1
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Near-infrared (NIR) core–shell CdSeTe/CdS quantum dots (QDs) modified with l-cysteine were synthesized in aqueous solution. The QDs had a special NIR-emitting spectrum, high fluorescence stability and low cytotoxicity. In addition, they exhibited an obvious fluorescence quenching when Cu2+ was present. An NIR nanosensor was prepared for rapidly, sensitively, and selectively determining Cu2+ in solution quantitatively and monitoring the changes in Cu2+ in cells with fluorescence imaging in a semiquantitative way. The linear relationship between the relative fluorescence intensity (F0/F) and the concentration of Cu2+ from 5.12 × 10−8 M to 2.56 × 10−5 M in solution was observed using an NIR fluorescence spectrophotometer with R2 equal to 0.9958. Moreover, in the experiment with the fluorescence microscope, F0/F versus the concentration of Cu2+ from 5.00 × 10−8 M to 7.68 × 10−6 M also showed a good linear relationship with R2 equal to 0.9817. Practical water sample ion detecting experiments had good accuracy and recovery rates. Cell experiments showed that the NIR imaging intensity of cells was inversely proportional to the concentration of copper ions, therefore NIR QDs have great potential for detection of metal ions in solution and in cells.
中文翻译：
用近红外 l-半胱氨酸封端的 CdSeTe/CdS 量子点测定 Cu2+ 及其对细胞成像的影响
在水溶液中合成了用l-半胱氨酸修饰的近红外 (NIR) 核壳 CdSeTe/CdS 量子点 (QD) 。量子点具有特殊的近红外发射光谱、高荧光稳定性和低细胞毒性。此外，当Cu 2+存在时，它们表现出明显的荧光猝灭。的NIR纳米传感器被用于快速，灵敏，和选择性地确定制备的Cu 2+在溶液定量和监测在Cu中的变化2+以半定量的方式与荧光成像中的细胞。相对荧光强度（F 0 /F）与Cu 2+浓度从5.12 × 10 -8 M 到2.56 × 10的线性关系 使用R 2等于0.9958的NIR荧光分光光度计观察溶液中的-5 M。此外，在荧光显微镜实验中，F 0 /F 与Cu 2+浓度从5.00 × 10 -8 M 到7.68 × 10 -6 M 也表现出良好的线性关系，R 2等于0.9817。实际水样离子检测实验具有良好的准确度和回收率。细胞实验表明，细胞的近红外成像强度与铜离子的浓度成反比，因此近红外量子点在检测溶液和细胞中的金属离子方面具有很大的潜力。
更新日期：2021-05-28
中文翻译：
用近红外 l-半胱氨酸封端的 CdSeTe/CdS 量子点测定 Cu2+ 及其对细胞成像的影响
在水溶液中合成了用l-半胱氨酸修饰的近红外 (NIR) 核壳 CdSeTe/CdS 量子点 (QD) 。量子点具有特殊的近红外发射光谱、高荧光稳定性和低细胞毒性。此外，当Cu 2+存在时，它们表现出明显的荧光猝灭。的NIR纳米传感器被用于快速，灵敏，和选择性地确定制备的Cu 2+在溶液定量和监测在Cu中的变化2+以半定量的方式与荧光成像中的细胞。相对荧光强度（F 0 /F）与Cu 2+浓度从5.12 × 10 -8 M 到2.56 × 10的线性关系 使用R 2等于0.9958的NIR荧光分光光度计观察溶液中的-5 M。此外，在荧光显微镜实验中，F 0 /F 与Cu 2+浓度从5.00 × 10 -8 M 到7.68 × 10 -6 M 也表现出良好的线性关系，R 2等于0.9817。实际水样离子检测实验具有良好的准确度和回收率。细胞实验表明，细胞的近红外成像强度与铜离子的浓度成反比，因此近红外量子点在检测溶液和细胞中的金属离子方面具有很大的潜力。
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