Abstract

Electrospun nanofibers have a natural wound healing effect due to their similarity to the extracellular matrix (ECM). Nigella sativa oil, which has therapeutic properties, is used for a wide variety of applications in traditional medicine. The aim of this study was to investigate the release characteristic and wound healing performance of Nigella sativa oil (NSO) loaded polyurethane (PU) electrospun nanofibrous mats in wound dressing applications. In addition, the antibacterial activity and cytotoxicity of the electrospun mats were studied. Analyses using a scanning electron microscope (SEM) showed that PU/NSO nanofibrous mat with an average fiber diameter of 416 ± 66 nm were successfully fabricated. NSO was released at a maximum ratio of 30% from the electrospun mat, and the Korsmeyer-Peppas model was identified as best for determining the release mechanism. Significant antibacterial activity was observed against Staphylococcus aureus (90.26%) and Escherichia coli (95.75%). The developed PU/NSO nanofibrous mat increased the cell viability more than 100% in human umbilical vein endothelial cell line (HUVEC) cell line. The NSO loaded PU nanofibrous mat significantly promoted the wound healing process on a rat wound model, and its wound closure reached approximately 85% compared to the control groups on the 9^th day (p < 0.01). The results indicated PU/NSO nanofibrous mat is a suitable candidate for a wound dressing.

摘要

电纺纳米纤维由于与细胞外基质 (ECM) 相似，因此具有天然的伤口愈合效果。Nigella sativa油具有治疗特性，在传统医学中有广泛的应用。本研究的目的是研究黑种草的释放特性和伤口愈合性能。油（NSO）负载聚氨酯（PU）电纺纳米纤维垫在伤口敷料应用中。此外，还研究了电纺毡的抗菌活性和细胞毒性。使用扫描电子显微镜 (SEM) 进行分析表明，成功制造了平均纤维直径为 416 ± 66 nm 的 PU/NSO 纳米纤维垫。NSO 以 30% 的最大比例从电纺垫中释放，Korsmeyer-Peppas 模型被认为是确定释放机制的最佳模型。对金黄色葡萄球菌(90.26%) 和大肠杆菌具有显着的抗菌活性(95.75%)。开发的 PU/NSO 纳米纤维垫使人脐静脉内皮细胞系 (HUVEC) 细胞系的细胞活力增加了 100% 以上。的NSO加载PU纳米纤维垫显著促进伤口上的大鼠伤口模型愈合过程，并与在9对照组其伤口闭合达到约85％的^第天（p  <0.01）。结果表明 PU/NSO 纳米纤维垫是伤口敷料的合适候选者。