We studied the influence of sucrose applied in combination with different concentrations of penetrating cryoprotectants (DMSO, ethylene glycol, and glycerol) on the efficiency of cryopreservation of umbilical cord-derived multipotent stromal cells (MSC). The results indicate that these cells can be cryopreserved with the use of 5-10% DMSO or ethylene glycol with equal efficiency; addition of 0.2 M sucrose does not affect cell survival after thawing. The efficiency of glycerol as a cryoprotectant increases with increasing its concentration from 5 to 10%, but remains significantly lower than the efficiency of DMSO or ethylene glycol. Addition of sucrose to a final concentration of 0.2 M increases the efficiency of glycerol. The efficiency of combination of 10% glycerol and sucrose was comparable with that of combinations of DMSO and ethylene glycol with sucrose. The mechanism of the observed enhancement is apparently related to the influence of sucrose on the dynamic properties of the lipid membranes and facilitation of glycerol diffusion into the cells.

我们研究了蔗糖与不同浓度的穿透性冷冻保护剂（DMSO、乙二醇和甘油）联合应用对脐带衍生的多能基质细胞 (MSC) 冷冻保存效率的影响。结果表明，这些细胞可以在使用 5-10% DMSO 或乙二醇的情况下以相同的效率进行冷冻保存；添加 0.2 M 蔗糖不会影响解冻后的细胞存活。甘油作为冷冻保护剂的效率随着其浓度从 5% 增加到 10% 而增加，但仍显着低于 DMSO 或乙二醇的效率。添加蔗糖至 0.2 M 的最终浓度可提高甘油的效率。10％甘油和蔗糖组合的效率与DMSO和乙二醇与蔗糖组合的效率相当。观察到的增强机制显然与蔗糖对脂质膜动态特性的影响和促进甘油扩散进入细胞有关。