Snails identified as Triodopsis hopetonensis were collected (n = 18) from the University of Arkansas–Fayetteville campus in December 2018. Additional snails were collected in April 2019 (n = 9) and in September 2019 (n = 9). Kidney tissues were examined using light microscopy, and polysporocystic oocysts were observed. Sporulated oocysts (n = 2) measured 78 ± 3.4 μm × 76 ± 2.9 μm with an irregular oocyst residuum and contained an estimated 44–55 tetrazoic sporocysts. The sporocysts (n = 10) measured 13 ± 0.5 μm × 11 ± 1.5 μm with an indistinct, irregularly placed, sporocyst residuum and usually contained 4 sporozoites, although an octozoic variant was observed. DNA was extracted from the snail kidney tissues and used as a template for polymerase chain reaction (PCR). PCR was used to determine the infection status of the snails; 13 of 36 (36%) specimens were found to be infected with a new Klossia species, and only 3 (8%) of these infections were detected using light microscopy. The complete nuclear 18S ribosomal DNA (1,800 bp) and mitochondrial genomes (6,775 bp) were generated, and they differentiated this parasite from the type species Klossia helicina and support the description of this new Klossia species, Klossia razorbacki n. sp. This is the first Klossia species to be described from any North American snail.

蜗牛被确定为Triodopsis Hopetonensis于 2018 年 12 月从阿肯色大学费耶特维尔校区收集 (n = 18)。2019 年 4 月 (n = 9) 和 2019 年 9 月 (n = 9) 收集了更多蜗牛。使用光学显微镜检查肾脏组织，并观察到多孢子囊卵囊。孢子化卵囊 (n = 2) 大小为 78 ± 3.4 μm × 76 ± 2.9 μm，带有不规则的卵囊残留物，估计含有 44-55 个四生孢子囊。孢子囊 (n = 10) 的尺寸为 13 ± 0.5 μm × 11 ± 1.5 μm，具有模糊不清、不规则放置的孢子囊残留物，通常包含 4 个子孢子，尽管观察到了八生代变种。从蜗牛肾组织中提取 DNA 并用作聚合酶链反应 (PCR) 的模板。PCR用于确定蜗牛的感染状态；36 个样本中的 13 个 (36%) 被发现感染了新的Klossia物种，并且使用光学显微镜仅检测到这些感染中的 3 (8%) 种。生成了完整的核 18S 核糖体 DNA (1,800 bp) 和线粒体基因组 (6,775 bp)，他们将这种寄生虫与Klossia helicina类型物种区分开来，并支持对这种新的Klossia物种Klossia razorbackin的描述。sp. 这是从任何北美蜗牛中描述的第一个Klossia物种。