Coagulation is a critical pathogenic process in Staphylococcus aureus. Although the agglutination of S. aureus has been studied for a long time, the genes involved in this process are not completely clear. We performed tube agglutination and dynamic turbidimetry tests to identify novel genes involved in reduced plasma coagulation. A total of 15 genes were identified, including coa, clfA, vwbp, saeS, agrA, trpC, spdC, mroQ, cydA, qoxC, sucC, pyrP, menH, threonine aldolase, and truncated transposase for IS1272. The functions of these genes include bicomponent regulation, membrane transport, energy metabolism and biosynthesis, respectively. cydA, spdC, and mroQ genes were further studied by gene knockout and complementation. Results of gene knockout and complementation and real-time-qPCR proved that cydA, spdC, and mroQ genes are necessary for plasma coagulation. Furthermore, the survival ability of 7 day mice decreased significantly when cydA, spdC, and mroQ genes had been knocked out.

中文翻译：

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cydA、spdC 和 mroQ 是参与金黄色葡萄球菌血浆凝固的新基因

凝血是金黄色葡萄球菌的关键致病过程。尽管对金黄色葡萄球菌的凝集作用进行了很长时间的研究，但参与这一过程的基因并不完全清楚。我们进行了试管凝集和动态比浊法测试，以鉴定参与降低血浆凝固的新基因。共鉴定出15个基因，包括coa、clfA、vwbp、saeS、agrA、trpC、spdC、mroQ、cydA、qoxC、sucC、pyrP、menH、苏氨酸醛缩酶和IS1272 的截短转座酶。这些基因的功能分别包括双组分调节、膜转运、能量代谢和生物合成。通过基因敲除和互补进一步研究了cydA、spdC和mroQ基因。基因敲除和互补以及实时qPCR的结果证明cydA、spdC和mroQ基因是血浆凝固所必需的。此外，敲除cydA、spdC和mroQ基因后，7天小鼠的存活能力显着下降。