Crystal structures of a nicotine MATE transporter provide insight into its mechanism of substrate transport,FEBS Letters

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Crystal structures of a nicotine MATE transporter provide insight into its mechanism of substrate transport
FEBS Letters ( IF 3.0 ) Pub Date : 2021-05-29 , DOI: 10.1002/1873-3468.14136
Yoshiki Tanaka ₁ , Shigehiro Iwaki ₁ , Akira Sasaki ₁ , Tomoya Tsukazaki ₁

Affiliation

A transporter of the multidrug and toxic compound extrusion (MATE) family, Nicotiana tabacum MATE2 (NtMATE2), is located in the vacuole membrane of the tobacco plant root and is involved in the transportation of nicotine, a secondary or specialized metabolic compound in Solanaceae. Here, we report the crystal structures of NtMATE2 in its outward-facing forms. The overall structure has a bilobate V-shape with pseudo-symmetrical assembly of the N- and C-lobes. In one crystal structure, the C-lobe cavity of NtMATE2 interacts with an unidentified molecule that may partially mimic a substrate. In addition, NtMATE2-specific conformational transitions imply that an unprecedented movement of the transmembrane α-helix 7 is related to the release of the substrate into the vacuolar lumen.

中文翻译：

尼古丁 MATE 转运蛋白的晶体结构有助于深入了解其底物转运机制

烟草MATE2 (NtMATE2) 是多药和有毒化合物挤出 (MATE) 家族的转运蛋白，位于烟草植物根部的液泡膜中，参与尼古丁（茄科次级或专门代谢化合物）的转运。在这里，我们报告了 NtMATE2 向外形式的晶体结构。整体结构呈双叶V形，N叶和C叶伪对称组装。在一种晶体结构中，NtMATE2 的 C 瓣腔与可能部分模仿底物的不明分子相互作用。此外，NtMATE2 特异性构象转变意味着跨膜 α-螺旋 7 前所未有的运动与底物释放到液泡腔有关。

更新日期：2021-07-27

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