Neuroblastoma is a common malignant tumor in children, and patients often have a poor prognosis. Long noncoding RNAs (lncRNAs) are involved in the regulation of neuroblastoma progression. However, the regulatory effect of lncRNA differentiation antagonizing nonprotein coding RNA (DANCR) on neuroblastoma is still not clear. The expression levels of DANCR, miR-338-3p and β-1, 4-galactosyltransferase III (B4GALT3) were determined by quantitative real-time PCR. 3-(4, 5-dimethyl-2 thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, flow cytometry and transwell assays were used to evaluate the proliferation, apoptosis, migration and invasion abilities of neuroblastoma cells. Moreover, western blot analysis was performed to assess the levels of B4GALT3 and the proliferation, apoptosis and migration-related proteins. Besides, a dual-luciferase reporter assay was used to verify the interactions among DANCR, miR-338-3p and B4GALT3. Mice xenograft models were used to ascertain the effect of DANCR on neuroblastoma tumor growth in vivo. Our results revealed that DANCR was highly expressed in neuroblastoma tissues and cells, and its silencing impeded the progression of neuroblastoma cells. DANCR could interact with miR-338-3p. Knockdown of miR-338-3p recovered the inhibitory effect of DANCR knockdown on neuroblastoma progression. B4GALT3 was a target of miR-338-3p. B4GALT3 overexpression reversed the suppression effect of DANCR silencing on neuroblastoma progression. In-vivo experiments further confirmed that DANCR silencing inhibited neuroblastoma tumor growth. Our results indicated that DANCR promoted B4GALT3 expression to increase the proliferation, migration and invasion of neuroblastoma cells via sponging miR-338-3p, which provided a theoretical basis for the targeted therapy of neuroblastoma.

中文翻译：

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长链非编码 RNA 分化拮抗非蛋白质编码 RNA 通过海绵化 miR-338-3p 靶向 β-1, 4-半乳糖基转移酶 III 促进神经母细胞瘤细胞的增殖、侵袭和迁移。

神经母细胞瘤是儿童常见的恶性肿瘤，患者预后较差。长链非编码 RNA (lncRNA) 参与调节神经母细胞瘤的进展。然而，lncRNA分化拮抗非蛋白编码RNA（DANCR）对神经母细胞瘤的调节作用仍不清楚。通过定量实时 PCR 测定 DANCR、miR-338-3p 和 β-1, 4-半乳糖基转移酶 III (B4GALT3) 的表达水平。使用 3-(4, 5-dimethyl-2 thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide、流式细胞术和 transwell 测定法评估神经母细胞瘤细胞的增殖、凋亡、迁移和侵袭能力。此外，进行蛋白质印迹分析以评估 B4GALT3 和增殖、凋亡和迁移相关蛋白的水平。除了，使用双荧光素酶报告基因测定来验证 DANCR、miR-338-3p 和 B4GALT3 之间的相互作用。小鼠异种移植模型用于确定 DANCR 对体内神经母细胞瘤肿瘤生长的影响。我们的研究结果表明，DANCR 在神经母细胞瘤组织和细胞中高表达，其沉默阻碍了神经母细胞瘤细胞的进展。DANCR 可以与 miR-338-3p 相互作用。敲低 miR-338-3p 恢复了敲低 DANCR 对神经母细胞瘤进展的抑制作用。B4GALT3 是 miR-338-3p 的靶标。B4GALT3 过表达逆转了 DANCR 沉默对神经母细胞瘤进展的抑制作用。体内实验进一步证实了 DANCR 沉默抑制了神经母细胞瘤的生长。我们的结果表明 DANCR 促进 B4GALT3 表达以增加增殖，