Genetic manipulation techniques for marine protists are not well-established, despite immense efforts. However, Perkinsus marinus is an exception and can be developed as a genetically tractable model organism for related protists. Here, we designed a new plasmid for P. marinus that allows two proteins from a single mRNA to be differently localized using a self-cleaving 2A peptide. This enabled us to establish a stable transfectant expressing a mitochondrially targeted fluorescent protein. The system can be applied to any protein in theory and would make a powerful tool for investigating unique organelles in P. marinus and related dinoflagellates.

中文翻译：

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一种新的含有 2A 肽的质粒，可产生表达细胞器靶向基因的稳定 Perkinsus marinus 细胞

尽管付出了巨大的努力，但用于海洋原生生物的基因操作技术尚未完善。然而，Perkinsus marinus是一个例外，可以开发为相关原生生物的遗传易处理模式生物。在这里，我们为P设计了一个新的质粒。 marinus允许来自单个 mRNA 的两种蛋白质使用自切割 2A 肽进行不同的定位。这使我们能够建立表达线粒体靶向荧光蛋白的稳定转染子。该系统理论上可以应用于任何蛋白质，并将成为研究P 中独特细胞器的强大工具。 marinus和相关的甲藻。