Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.

中文翻译：

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从粪便中快速制备胃肠道线虫卵用于 PCR 鉴定

将胃肠线虫 (GIN) 检测作为定性和定量测试是非常可取的。多重和 qPCR 等方法能够提供此类结果，但既费力又昂贵。本文介绍了一种从粪便样本中制备 GIN 鸡蛋的快速、低成本的方法，该方法可产生适用于 PCR分析的DNA 。我们还描述了一组适用于单管多重 PCR 的引物。