Lung transplantation is one of the most difficult and delicate procedures among organ transplants. For the success of the procedure and survival of the new organ, the sterilization step for acellular lungs prior to recellularization is important to ensure that they are free of any risk of transmitting infections from the donor to the recipient subject. However, there are no available information concerning the lung mechanical parameters after sterilizing photodynamic therapy. The aim of this study was to evaluate the extracellular matrix (ECM) and lung mechanical parameters of decellularized lungs undergoing sterilizing photodynamic therapy (PDT). Besides, we also analyzed the lung after controlled infection with C. albicans in order to evaluate the effectiveness of PDT. The lung mechanical evaluation parameters, resistance () and elastance (), exhibited no significant differences between groups. In addition, there were no PDT-induced changes in lung properties, with maintenance of the viscoelastic behavior of the lung scaffold after 1 h exposure to PDT. The ECM components remained virtually unchanged in the acellular lungs of both groups. We also showed that there was a reduction in fungal infection population after 45 minutes of PDT. However, more studies should be performed to establish and verify the effectiveness of PDT as a possible means for sterilizing lung scaffolds. This manuscript was presented as a master thesis of Nadua Apostólico at the postgraduate program in rehabilitation sciences, University Nove de Julho—UNINOVE.

中文翻译：

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小鼠肺细胞外基质中的光动力疗法：替代组织灭菌过程的初步结果

肺移植是器官移植中最困难、最精细的手术之一。为了成功完成该程序并确保新器官的存活，在重新细胞化之前对无细胞肺进行灭菌的步骤非常重要，以确保它们没有任何将感染从供体传播到接受对象的风险。然而，没有关于灭菌光动力疗法后肺机械参数的可用信息。本研究的目的是评估接受光动力疗法 (PDT) 的脱细胞肺的细胞外基质 (ECM) 和肺机械参数。此外，我们还分析了控制感染白色念珠菌后的肺以评估 PDT 的有效性。肺力学评价参数，阻力（）和弹性（），组间无显着差异。此外，在暴露于 PDT 1 小时后，肺支架的粘弹性行为保持不变，没有 PDT 诱导的肺特性变化。ECM 成分在两组的脱细胞肺中几乎保持不变。我们还显示，PDT 45分钟后真菌感染种群减少。然而，应该进行更多的研究来建立和验证 PDT 作为对肺支架进行消毒的可能手段的有效性。这份手稿作为 Nadua Apostólico 的硕士论文在新儒略大学 — UNINOVE 康复科学研究生课程中提交。