Due to its good biocompatibility and degradability, magnesium alloy (Mg alloy) has shown great promise in cardiovascular stent applications. Rapid stent re-endothelialization is derived from migrated and adhered endothelial cells (ECs), which is an effective way to reduce late thrombosis and inhibit hyperplasia. However, fundamental questions regarding Mg alloy affecting migration and adhesion of ECs are not fully understood. Here, we evaluated the effects of Mg alloy on the ECs proliferation, adhesion and migration. A global gene expression profiling of ECs co-culturing with Mg alloy was conducted, and the adhesion- and migration-related genes were examined. We found that Mg alloy had no adverse effects on ECs viability but significantly affected ECs migration and adhesion. Co-cultured with Mg alloy extract, ECs showed contractive adhesion morphology and decreased motility, which was supported by the down-regulation of adhesion-related genes (Paxillin and Vinculin) and migration-related genes (RAC 1, Rho A and CDC 42). Accordingly, the re-endothelialization of Mg alloy stent was inhibited in vivo. Our results may provide new inspiration for improving the broad application of Mg alloy stents.

中文翻译：

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内皮细胞对镁合金的生物学反应及机制

由于其良好的生物相容性和可降解性，镁合金（Mg合金）在心血管支架应用中显示出巨大的前景。快速支架再内皮化源自迁移和粘附的内皮细胞（EC），是减少晚期血栓形成和抑制增生的有效途径。然而，有关镁合金影响 EC 迁移和粘附的基本问题尚未完全了解。在这里，我们评估了镁合金对 ECs 增殖、粘附和迁移的影响。对与镁合金共培养的 ECs 进行了全局基因表达谱分析，并检查了粘附和迁移相关基因。我们发现镁合金对 ECs 的活力没有不利影响，但显着影响 ECs 的迁移和粘附。与镁合金提取物共培养时，ECs表现出收缩性粘附形态和运动性下降，这得到了粘附相关基因（桩蛋白和粘蛋白）和迁移相关基因（RAC 1、Rho A和CDC 42）下调的支持。 。因此，镁合金支架的再内皮化在体内受到抑制。我们的研究结果可能为改善镁合金支架的广泛应用提供新的启发。