The present study investigated the effect of plasma-produced reactive oxygen (ROS) and nitrogen (RNS) species on cancer cell viability. Reactive species were generated in deionized water by using an atmospheric pressure Ar plasma jet within the controlled ambient gases (air, O₂ or N₂), which allowed the production of plasma-activated water containing only ROS (e.g. O₃, H₂O₂) or both ROS and RNS (e.g. H₂O₂, NO₂^–, NO₃^–). A considerable amount of H₂O₂ was produced in all ambient gases, and its generation rate was highest in N₂ and lowest in O₂. The latter was connected with a H₂O₂ precursor, OH, efficient quenching in O₂ ambient gas. Small quantities of NO₂^– were generated during short (< 5 min) plasma treatments in ambient air and N₂. The highest amount of NO₃^– was produced in N₂ ambient gas. Ozone was detected only in the case of O₂ environment. Cell viability studies were carried out by utilizing two cancer cell lines: 4T1 (breast cancer) and PPC-1 (prostate cancer). The results of the colorimetric succinate dehydrogenase activity assay showed that the studied cell lines had a similar sensitivity to the plasma activated medium. The impact of medium produced in the O₂ ambient environment was determined by H₂O₂ content. The equivalent amount of H₂O₂ in the plasma activated medium produced in the N₂ ambient environment caused an almost two-fold higher viability than in the case of the O₂ ambient gas. It is proposed that this was due to the cellular proliferation enhancing effect of NH₃.

本研究调查了等离子体产生的活性氧 (ROS) 和氮 (RNS) 物种对癌细胞活力的影响。通过在受控环境气体（空气、O ₂或 N ₂）内使用常压 Ar 等离子体射流在去离子水中生成活性物质，这允许生产仅包含 ROS（例如 O ₃、H ₂ O）的等离子体活化水₂ ) 或 ROS 和 RNS (例如 H ₂ O ₂ , NO ₂ ^– , NO ₃ ^– )。在所有环境气体中都会产生大量的H ₂ O ₂，并且其生成速率在N中最高₂和最低的 O ₂。后者与 H ₂ O ₂前体 OH 连接，在 O ₂环境气体中有效淬火。在环境空气和 N _{2 中}进行短时间（< 5 分钟）等离子处理期间会产生少量 NO ₂ ^–。在 N ₂环境气体中产生的 NO ₃ ^–量最高。仅在 O ₂的情况下检测到臭氧环境。通过利用两种癌细胞系进行细胞活力研究：4T1（乳腺癌）和 PPC-1（前列腺癌）。比色琥珀酸脱氢酶活性测定的结果表明所研究的细胞系对血浆活化培养基具有相似的敏感性。O ₂周围环境中产生的介质的影响由H ₂ O ₂含量确定。在 N ₂周围环境中产生的等离子活化介质中的等量 H ₂ O ₂导致比在 O ₂周围气体情况下的存活率高几乎两倍。推测这是由于NH的细胞增殖增强作用₃ .