Treatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections. PdB was prepared through the freezing and thawing process and then, in vitro antibacterial effect was determined by disk diffusion and broth microdilution methods. Afterward, burn wound was inflicted on 56 rats, infected with both bacteria, and topical administration was performed to evaluate antibacterial effects and wound healing properties of PdB. In vitro results showed that PdB inhibited the growth of A. baumannii in the highest dose (0.5), while we did not detect any inhibitory effects against K. pneumoniae. By contrast, PdB significantly inhibited the growth of bacteria in treated animal wounds compared to the control groups (P value < 0.05). Macroscopic assessments pointed to the significant enhancement of wound closure in the treated animals. In addition, histopathological examination demonstrated that treatment of rats with PdB led to a considerable increase in re-epithelialization and attenuated the formation of granulation tissue (P value < 0.05). The use of topical PdB is an attractive strategy for treating A. baumannii and K. pneumoniae burn wound infections because it inhibits bacterial growth and promotes wound healing properties.

中文翻译：

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血小板裂解液对鲍曼不动杆菌和肺炎克雷伯菌烧伤伤口感染的抗菌和伤口愈合特性研究

由于多重耐药菌的传播，烧伤伤口感染的治疗已成为全球性挑战；因此，为上述感染开发新的治疗方案至关重要。血小板为此目的引起了很多关注，因为它们是不同抗菌肽和生长因子的安全且具有成本效益的来源。本研究评估了血小板衍生生物材料 (PdB) 对鲍曼不动杆菌和肺炎克雷伯菌烧伤伤口感染的抗菌作用和伤口愈合特性。PdB通过冻融过程制备，然后通过圆片扩散法和肉汤微量稀释法测定体外抗菌效果。之后，56 只老鼠被烧伤，感染了两种细菌，并进行局部给药以评估 PdB 的抗菌作用和伤口愈合特性。体外结果表明，PdB 以最高剂量 (0.5) 抑制鲍曼不动杆菌的生长，而我们没有检测到对肺炎克雷伯菌的任何抑制作用。相比之下，与对照组相比，PdB 显着抑制了治疗动物伤口中细菌的生长（P 值 < 0.05）。宏观评估表明治疗动物的伤口闭合显着增强。此外，组织病理学检查表明，用 PdB 治疗大鼠导致上皮再形成显着增加并减弱肉芽组织的形成（P 值 < 0.05）。使用局部 PdB 是治疗鲍曼不动杆菌和 K.