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Effects of black soldier fly pulp on growth performance, histomorphology and lipid metabolism gene expression of Micropterus salmoides
Aquaculture Reports ( IF 3.2 ) Pub Date : 2021-05-27 , DOI: 10.1016/j.aqrep.2021.100737
Kai Peng , Wenyan Mo , Hongfa Xiao , Guoxia Wang , Yanhua Huang

A 62-day feeding trial was conducted to assess the effects of dietary black soldier fly pulp (BSFP) on growth performance, liver and intestine histomorphology, and liver lipid metabolism gene expression of Micropterus salmoides. Four isoproteic and isolipidic diets were formulated by adding BSFP (original substance) to the basal diet at the rate of 0% (BSFP0), 1% (BSFP1), 2% (BSFP2) and 4% (BSFP4), corresponding to inclusion of 0, 4.5, 9.0 and 18.0 g/kg DM in diets. Each diet was randomly assigned to triplicate groups of 35 fish per tank. Fish were fed twice daily to apparent satiation. Results indicated that fish fed BSFP2 had higher (p < 0.05) weight gain rate, specific growth rate and liver lipid content than BSFP0. Hepatocytes were observed to fill with lipids among treatments with different degrees of vacuolar degeneration and inflammatory cell infiltration in BSFP treated diets. Intestinal villus length was lower (p < 0.05) in BSFP2 and BSFP4 than in other diets, and villus width was lower (p < 0.05) in BSFP4 than BSFP0. Gene expression of peroxisome proliferators-activated receptors α and lipoprotein lipase were down-regulated (p < 0.05) with increased dietary BSFP levels. Acetyl-CoA carboxylase 1 was higher (p < 0.05) in fish fed BSFP4 than other diets. It was concluded that dietary inclusion of BSFP increased specific growth rate and lipid deposition, and included inflammation or damage in the liver and intestine of Micropterus salmoides.



中文翻译:

黑水虻果肉对鲑鱼生长性能、组织形态及脂质代谢基因表达的影响

A 62天喂养试验以评估饮食黑色士兵蝇纸浆(BSFP)生长性能,肝和肠组织形态学,和肝脏脂质代谢的基因表达的影响加州鲈. 通过将 BSFP(原始物质)以 0% (BSFP0)、1% (BSFP1)、2% (BSFP2) 和 4% (BSFP4) 的比例添加到基础日粮中,配制了四种等蛋白和等脂日粮,对应于包含饮食中 0、4.5、9.0 和 18.0 g/kg DM。每种饮食被随机分配到每缸 35 条鱼的三份重复组中。每天喂鱼两次以达到明显的饱腹感。结果表明,与 BSFP0 相比,饲喂 BSFP2 的鱼具有更高的(p < 0.05)增重率、比生长率和肝脏脂质含量。在 BSFP 处理的饮食中,在不同程度的空泡变性和炎性细胞浸润的处理中,观察到肝细胞充满脂质。BSFP2 和 BSFP4 中的肠绒毛长度低于其他饮食(p < 0.05),而 BSFP4 中的绒毛宽度低于 BSFP0(p < 0.05)。过氧化物酶体增殖物激活受体 α 和脂蛋白脂肪酶的基因表达随着膳食 BSFP 水平的增加而下调(p < 0.05)。与其他饮食相比,饲喂 BSFP4 的鱼的乙酰辅酶 A 羧化酶 1 更高(p < 0.05)。结论是饮食中包含 BSFP 增加了特定的生长速度和脂质沉积,并包括肝脏和肠道的炎症或损伤。鲑鱼

更新日期:2021-05-28
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