The biological processes of Chlamys farreri (C. farreri), an economically important shellfish, are affected when exposed to Cd²⁺. In this study, changes to biological processes and metabolite levels in C. farreri were examined when exposed to Cd²⁺. Ultra-performance liquid chromatography-tandem TOF mass spectrometry (UPLC-TOF/MS)-based untargeted metabolomics was used to examine changes in the metabolism of C. farreri gill tissue exposed to 0.050 mg/L Cd²⁺ for 96 h in a natural environment. Sixty-eight metabolites with significant differences were screened by multivariate statistical analysis. Eleven enriched functional pathways displayed significant changes in inactivity. Differential metabolites, mainly C00157 and C00350, have a significant impact on functional pathways and can be used as potential major biomarkers. Lipid phosphorylation, disruption of signal transduction, and autophagy activation were observed to change in C. farreri when exposed to Cd. The metabolome information supplements research on C. farreri exposure to heavy metals and provides a platform for further multi-omics analysis.

当接触 Cd ²⁺时，Chlamys farreri ( C. farreri ) 是一种具有重要经济意义的贝类，其生物过程会受到影响。在这项研究中，当接触 Cd ²⁺时，检查了C. farreri生物过程和代谢物水平的变化。使用基于超高效液相色谱-串联 TOF 质谱 (UPLC-TOF/MS) 的非靶向代谢组学检测暴露于 0.050 mg/L Cd ²⁺的C. farreri鳃组织的代谢变化在自然环境中保持 96 小时。通过多变量统计分析筛选出具有显着差异的68种代谢物。11 条丰富的功能通路显示出不活动的显着变化。差异代谢物，主要是 C00157 和 C00350，对功能通路有显着影响，可用作潜在的主要生物标志物。当暴露于 Cd 时，在C. farreri中观察到脂质磷酸化、信号转导中断和自噬激活发生变化。代谢组信息补充了对C. farreri重金属暴露的研究，并为进一步的多组学分析提供了平台。