CyberKnife radiation therapy as a platform for translational mouse studies,International Journal of Radiation Biology

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CyberKnife radiation therapy as a platform for translational mouse studies
International Journal of Radiation Biology ( IF 2.6 ) Pub Date : 2021-06-23 , DOI: 10.1080/09553002.2021.1934749
Martha Kiljan _{1,

2,

3} , Sabrina Weil _{2,

3,

4} , Andres Vásquez-Torres _{1,

3} , Meike Hettich _{1,

3} , Marimel Mayer _{1,

3} , Olta Ibruli _{2,

3,

4} , Matthias Reinscheid _{1,

2,

3} , Isabelle Heßelmann _{1,

2,

3} , Jiali Cai _{1,

2,

3} , Li-Na Niu _{1,

2,

3} , Yagmur Sahbaz _{2,

3,

4} , Christian Baues _{1,

4} , Wolfgang W Baus _{1,

3} , Florian Kamp _{1,

3} , Simone Marnitz _{1,

3} , Grit S Herter-Sprie _{2,

3,

4} , Jan M Herter _{1,

2,

3}

Affiliation

Abstract

Purpose

Radiation therapy (RT) is a common nonsurgical treatment in the management of patients with cancer. While genetically engineered mouse models (GEMM) recapitulate human disease, conventional linear particle accelerator systems are not suited for state-of-the-art, imageguided targeted RT (IGRT) of these murine tumors. We employed the CyberKnife (CK; Accuray) platform for IGRT of GEMM-derived non-small cell lung cancer (NSCLC) lesions.

Material and methods

GEMM-derived Kras^LSL-G12D/+/Trp53^fl/fl -driven NSCLC flank tumors were irradiated using the CK RT platform. We applied IGRT of 2, 4, 6, and 8 Gy using field sizes of 5–12.5 mm to average gross tumor volumes (GTV) of 0.9 cm3 using Xsight Spine Tracking (Accuray).

Results

We found that 0 mm planning target volume (PTV) margin is sufficient for IGRT of murine tumors using the CK. We observed that higher RT doses (6–8 Gy) decreased absolute cell numbers of tumor infiltrating leukocytes (TIL) by approximately half compared to low doses (2–4 Gy) within 1 h, but even with low dose RT (2 Gy) TIL were found to be reduced after 8–24 h.

Conclusion

We here demonstrate that the CK RT system allows for targeted IGRT of murine tumors with high precision and constitutes a novel promising platform for translational mouse RT studies.

中文翻译：

CyberKnife 放射治疗作为转化小鼠研究的平台

摘要

目的

放射治疗 (RT) 是治疗癌症患者的常见非手术治疗方法。虽然基因工程小鼠模型 (GEMM) 概括了人类疾病，但传统的线性粒子加速器系统不适合这些鼠类肿瘤的最先进的图像引导靶向 RT (IGRT)。我们采用射波刀 (CK; Accuray) 平台对 GEMM 衍生的非小细胞肺癌 (NSCLC) 病变进行 IGRT。

材料与方法

使用 CK RT 平台照射GEMM 衍生的 Kras ^LSL-G12D/+ /Trp53 ^fl/fl驱动的 NSCLC 侧腹肿瘤。我们使用 Xsight Spine Tracking (Accuray) 应用 2、4、6 和 8 Gy 的 IGRT，使用 5–12.5 mm 的野大小，平均总肿瘤体积 (GTV) 为 0.9 cm3。

结果

我们发现 0 mm 的计划靶体积 (PTV) 边缘足以使用 CK 对小鼠肿瘤进行 IGRT。我们观察到，与低剂量 (2-4 Gy) 相比，较高的放疗剂量 (6–8 Gy) 在 1 小时内将肿瘤浸润性白细胞 (TIL) 的绝对细胞数减少了大约一半，但即使是低剂量放疗 (2 Gy)发现 TIL 在 8-24 小时后降低。