The study focuses on a non-stringent, rapid and sustainable way for the synthesis of silver oxide nanoparticles (Ag₂ONPs) using aqueous rhizome extract of Curcuma zanthorrhiza Roxb. (Cz). High resolution liquid chromatography mass spectroscopy (HR-LCMS) was used for the simultaneous identification of bioactive molecules in the aqueous rhizome extract and its biosynthesized nanoparticles. The presence of eleven bioactive molecules in the rhizome extract acts as reducing and capping agents during the synthesis of Ag₂ONPs. The molecules coated to Ag₂ONPs were identified to be majorly sesquiterpenoids and lipid molecules. The analytical techniques used for the nanoparticle characterization included UV–Visible spectrum, which showed SPR band at 409 nm; FTIR spectrum depicted the bioactive molecules involved in capping and reduction of silver ions to silver oxide nanoparticles; XRD pattern attributed to fcc structure of CzAg₂ONPs with an average size of 39.7 nm; HR-TEM and FESEM confirmed the size and morphology of CzAg₂ONPs. The chemical nature of the bioactive molecules bound to Ag₂ONPs revealed by HR-LCMS was in agreement with FTIR spectral data. The CzAg₂ONPs exhibited efficient photocatalytic activity in the degradation of the toxic dye malachite green (MG) as revealed by the absorption spectra. The degraded product was subjected to HR-LCMS and found to be non-toxic. The results revealed the promising potential of bioactive molecules coated Ag₂ONPs for environmental cleanup.

该研究的重点是一种非严格、快速和可持续的方法，用于使用姜黄根茎提取物合成氧化银纳米粒子 (Ag ₂ ONPs)。(Cz)。高分辨率液相色谱质谱 (HR-LCMS) 用于同时鉴定根茎水提取物及其生物合成纳米颗粒中的生物活性分子。根茎提取物中存在的 11 个生物活性分子在 Ag ₂ ONPs的合成过程中充当还原剂和封端剂。涂在Ag _2上的分子ONPs 被确定为主要是倍半萜类化合物和脂质分子。用于纳米颗粒表征的分析技术包括紫外-可见光谱，在 409 nm 处显示 SPR 带；FTIR 光谱描绘了参与将银离子封闭和还原为氧化银纳米颗粒的生物活性分子；XRD 图归因于平均尺寸为 39.7 nm的 CzAg ₂ ONPs 的 fcc 结构；HR-TEM 和 FESEM 证实了 CzAg ₂ ONPs 的尺寸和形态。HR-LCMS 揭示的与 Ag ₂ ONPs结合的生物活性分子的化学性质与 FTIR 光谱数据一致。CzAg ₂如吸收光谱所示，ONPs 在降解有毒染料孔雀石绿 (MG) 中表现出有效的光催化活性。降解产物经过HR-LCMS检测，无毒。结果揭示了生物活性分子包覆的 Ag ₂ ONPs 在环境净化方面的潜力。