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Using cytogenetic analysis to identify the genetic diversity in Lilium hansonii (Liliaceae), an endemic species of Ulleung Island, Korea
Horticulture, Environment, and Biotechnology ( IF 2.5 ) Pub Date : 2021-05-28 , DOI: 10.1007/s13580-021-00350-1
Yu-Hyeon Choi , Fahad Ramzan , Yoon-Jung Hwang , Adnan Younis , Chang-Kil Kim , Ki-Byung Lim

In section Martagon of genus Lilium, L. hansonii is an endemic species of Ulleung island, Korea. Deep golden yellow flowers and two to four whorls of leaves distinguish L. hansonii from other Martagon species. This species is declining in population due to destruction of the wild habitat. Here, we report comprehensive cytogenetic research on 37 individuals of L. hansonii using fluorescence in situ hybridization (FISH). Results of the FISH analysis showed that the distribution of 5S ribosomal DNAs was identical among all individuals of L. hansonii, but numbers of loci of 45S rDNA varied from 13 to 17 and 11 45S rDNA patterns were detected. Based on population level, 45S rDNA loci 13 and 17 were only detected in Seonginbong and Nariryeong, respectively. In addition, certain types of 14 and 15 45S rDNAs loci were found only in Seonginbong and Nariryeong, respectively. In contrast to 45S rDNA, a pair of 5S rDNAs on chromosome 3 was detected in all individuals consistently. The results revealed high genetic diversity in L. hansonii and will help establish a conservation strategy for this species.



中文翻译:

使用细胞遗传学分析鉴定韩国郁陵岛特有种百合(百合科)的遗传多样性

百合属的Martagon科中,L. hansonii是韩国郁陵岛的特有种。深金黄色的花朵和两到四片叶轮将L. hansonii与其他Martagon物种区分开来。由于野生栖息地的破坏,该物种的数量正在减少。在这里,我们报告了使用荧光原位杂交 (FISH)对 37 个乳杆菌进行的全面细胞遗传学研究。FISH分析结果表明,乳杆菌所有个体的5S核糖体DNA分布相同,但 45S rDNA 的基因座数量从 13 到 17 不等,检测到 11 45S rDNA 模式。根据种群水平,45S rDNA 基因座 13 和 17 分别仅在 Seonginbong 和 Nariryeong 检测到。此外,某些类型的 14 和 15 45S rDNAs 位点分别仅在 Seonginbong 和 Nariryeong 中发现。与 45S rDNA 相比,在所有个体中一致检测到 3 号染色体上的一对 5S rDNA。结果显示L. hansonii具有很高的遗传多样性,将有助于为该物种制定保护策略。

更新日期:2021-05-28
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