Biofilms play a key role in infectious diseases, as they may form on the surface and persist after treatment with various antimicrobial agents. The Staphylococcus aureus, Klebsiella pneumoniae, S. typhimurium, P. aeruginosa, and Escherichia coli most frequently associated with medical devices. Chitosan sulphate from marine litter (SCH-MW) was extracted and the mineral components were determined using atomic absorption spectroscopy (AAS). The degree of deacetylation (DA) of SCH was predicted 50% and 33.3% in crab and shrimp waste respectively. The elucidation of the structure of the SCH-MW was portrayed using FT-IR and 1H-NMR spectroscopy. The molecular mass of SCH-MW was determined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). The teratogenicity of SCH-MW was characterized by the zebrafish embryo (ZFE) model. Antimicrobial activity of SCH-MW was tested with the agar well diffusion method; the inhibitory effect of SCH-MW on biofilm formation was assessed in 96 flat well polystyrene plates. The result revealed that a low concentration of crab-sulfated chitosan inhibited bacterial growth and significantly reduced the anti-biofilm activity of gram-negative and gram-positive bacteria relatively to shrimp. It is potentially against the biofilm formation of pathogenic bacteria.

生物膜在传染病中起着关键作用，因为它们可能在表面形成并在用各种抗菌剂处理后持续存在。的金黄色葡萄球菌，肺炎克雷伯氏菌，鼠伤寒沙门氏菌，绿脓杆菌，和大肠杆菌最常与医疗装置相关联。从海洋垃圾中提取壳聚糖硫酸盐 (SCH-MW)，并使用原子吸收光谱 (AAS) 测定矿物成分。预计蟹和虾废物中 SCH 的脱乙酰度 (DA) 分别为 50% 和 33.3%。的SCH-MW的结构的阐明被描绘使用 FT-IR 和 1H-NMR 光谱。SCH-MW 的分子量是用基质辅助激光解吸/电离飞行时间 (MALDI-TOF) 确定的。SCH-MW 的致畸性由斑马鱼胚胎 (ZFE) 模型表征。用琼脂孔扩散法检测SCH-MW的抗菌活性；在 96 平孔聚苯乙烯板中评估了 SCH-MW 对生物膜形成的抑制作用。结果表明，相对于虾，低浓度的蟹硫酸化壳聚糖抑制细菌生长并显着降低革兰氏阴性和革兰氏阳性菌的抗生物膜活性。它潜在地对抗病原菌的生物膜形成。