Soil salinity is one of the most critical environmental stresses that affects crop productivity. In a context in which world demand for food is growing continuously, this problem requires urgent attention. Actions that go beyond traditional agricultural practices are needed. The objective of the current study was to develop a bioactive, economic, and sustainable compound that can increase the tolerance of cultivated plants in saline-stress situations by combining the hosting capacity of natural bentonite nanoclay (Bent) with a phytoactive osmoprotective compound, L-Proline (Pro). The Bent-Pro nanocomposite synthesis method, its final chemical structure, and in vitro bioactivity were addressed here. The results indicated that Bent can retain a maximum of 14.4% (w/w) of Pro. The (001) X-ray diffraction (XRD) peak of Bent shifted to smaller angles in the pattern of Bent-Pro, indicating that Pro has a monolayer arrangement between the Bent layers. The results of transmission electron microscopy (TEM) also supported this result. Pro was also retained on the edges or external surfaces of Bent, as indicated by thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). In addition, Pro functional groups identified by Fourier-transform infrared (FTIR) spectroscopy indicated that it was present in its zwitterionic form. The role of Bent-Pro as a protector against plant saline stress was assayed using Arabidopsis thaliana (A. thaliana) as a model, demonstrating that it mitigates the detrimental effects of NaCl-mediated salt stress on seed germination and the leaf chlorophyll level, thus highlighting the relevance of this contribution and the versatility and broad applicability of clays.

土壤盐分是影响作物生产力的最关键的环境压力之一。在世界粮食需求持续增长的背景下，这个问题亟待关注。需要采取超越传统农业做法的行动。本研究的目的是开发一种生物活性、经济和可持续的化合物，通过将天然膨润土纳米粘土 (Bent) 的承载能力与植物活性渗透保护化合物 L-相结合，提高栽培植物在盐胁迫条件下的耐受性。脯氨酸 (Pro)。Bent-Pro 纳米复合材料的合成方法、其最终化学结构和体外生物活性都在此处进行了讨论。结果表明，Bent 最多可保留 14.4% (w/w) 的 Pro。Bent 的 (001) X 射线衍射 (XRD) 峰在 Bent-Pro 的图案中向更小的角度移动，表明 Pro 在 Bent 层之间具有单层排列。透射电子显微镜 (TEM) 的结果也支持了这一结果。热重分析 (TGA) 和扫描电子显微镜 (SEM) 表明，Pro 还保留在 Bent 的边缘或外表面。此外，通过傅里叶变换红外 (FTIR) 光谱鉴定的 Pro 官能团表明它以两性离子形式存在。Bent-Pro 作为植物盐胁迫保护剂的作用进行了分析。热重分析 (TGA) 和扫描电子显微镜 (SEM) 表明，Pro 还保留在 Bent 的边缘或外表面。此外，通过傅里叶变换红外 (FTIR) 光谱鉴定的 Pro 官能团表明它以两性离子形式存在。Bent-Pro 作为植物盐胁迫保护剂的作用进行了分析。热重分析 (TGA) 和扫描电子显微镜 (SEM) 表明，Pro 还保留在 Bent 的边缘或外表面。此外，通过傅里叶变换红外 (FTIR) 光谱鉴定的 Pro 官能团表明它以两性离子形式存在。使用以下方法分析了Bent-Pro作为植物盐胁迫保护剂的作用：拟南芥( A. thaliana ) 作为模型，证明它减轻了 NaCl 介导的盐胁迫对种子萌发和叶片叶绿素水平的不利影响，从而突出了这种贡献的相关性以及粘土的多功能性和广泛适用性。