The One Health approach is not only focused on diseases and zoonosis control but also on antimicrobial resistance. As concern this important issue, the problem of plasmid-mediated colistin resistance recently emerged. Few studies reported data about colistin resistance and mcr genes in bacteria from wildlife. In this manuscript, 168 Escherichia coli isolated from hunted wild boar were tested; colistin resistance was evaluated by MIC microdilution method, and the presence of mcr-1 and mcr-2 genes was evaluated by PCR. Overall, 27.9% of isolates resulted resistant to colistin, and most of them showed a MIC value > 256 μg/mL. A percentage of 44.6% of tested E. coli scored positive for one or both genes. In details, 13.6% of isolated harbored mcr-1 and mcr-2 in combination; most of them exhibiting the highest MIC values. Interestingly, 19.6% of mcr-positive E. coli resulted phenotypically susceptible to colistin. Wild boar could be considered a potential reservoir of colistin-resistant bacteria. In the light of the possible contacts with domestic animals and humans, this wild species could play an important role in the diffusion of colistin resistance. Thus, the monitoring programs on wildlife should include this aspect.

One Health方法不仅关注疾病和人畜共患病的控制，而且还关注抗菌素的耐药性。关于这个重要问题，最近出现了质粒介导的粘菌素抗性的问题。很少有研究报告有关野生动物细菌中大肠菌素抗性和mcr基因的数据。在本手稿中，测试了从猎获的野猪中分离出的168株大肠杆菌。通过MIC微量稀释法评估大肠菌素抗性，并通过PCR评估mcr-1和mcr-2基因的存在。总体而言，有27.9％的分离株产生了对大肠菌素的抗性，其中大多数显示的MIC值> 256μg/ mL。被测大肠杆菌的百分比为44.6％一个或两个基因得分均为阳性。详细地讲，有13.6％的孤立的mcr-1和mcr-2组合在一起；他们大多数表现出最高的MIC值。有趣的是，19.6％的mcr阳性大肠杆菌在表型上对大肠杆菌素敏感。野猪可能被认为是抵抗大肠菌素的细菌的潜在库。鉴于可能与家畜和人类接触，这种野生物种可能在粘菌素抗性的扩散中起重要作用。因此，关于野生动植物的监测计划应包括这一方面。