Fatty acid composition and bioactivity of Sesbania sesban seed oils were analyzed. Seeds were milled by a domestic mixer at medium speed to obtain mixture containing germ and endosperm with seed coat. Germ (42.48%), Crude endosperm (26.22%) and seed coat (11.33%) were separated and whole seed along with seed parts subjected to extraction process by hot and cold method with petroleum ether. Saturated and unsaturated fatty acids in seed oils were determined and analyzed using Gas-liquid chromatography in which palmitic acid was found in endosperm oil (50.51%) and seed coat oil (30.65%) by cold extraction. Oleic acid was found in seed oil (48.22%) and in germ oil (46.87%) extracted by cold but lower in oils obtained by hot method. Linoleic acid content was found higher in oils extracted by hot extraction. Antioxidant activity was also done of oil from seed and their parts by different methods in which maximum inhibition shown by seed oil 55.45% at 100 μg/mL and minimum in endosperm oil 0.37% at all concentration from hot and cold method, which was significantly different at p<0.05 in the same column.The antimicrobial activity of seed oil was determined by disc diffusion method and the seed coat and whole seed extracted by cold extraction showed significant anti-bacterial activity. Studies reveal the potentiality of seed oil as an alternative source as/in edibles and also prospects the analysed oils of seed parts with useful bioactive markers for pharmaceuticals, nutraceuticals and cosmetics.

中文翻译：

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芝麻种子油的脂肪酸组成和生物活性

分析了Sesbania sesban种子油的脂肪酸组成和生物活性。用家用混合器将种子以中等速度碾磨，以获得带有种皮的含有胚芽和胚乳的混合物。分离出胚芽（42.48％），粗胚乳（26.22％）和种皮（11.33％），将整个种子以及种子部分通过石油醚的冷热法进行提取。使用气液色谱法测定并分析了种子油中的饱和和不饱和脂肪酸，其中通过冷萃取在胚乳油（50.51％）和种皮油（30.65％）中发现了棕榈酸。冷提取的种子油和胚芽油中的油酸含量分别为48.22％和46.87％，而热法油中的含量较低。发现通过热萃取提取的油中亚油酸含量更高。还通过不同的方法对种子及其部位的油进行了抗氧化活性测试，其中在100μg/ mL的条件下，种子油的最大抑制率为55.45％，而在所有浓度下，胚乳油的抑制作用最低，在热和冷方法下均为0.37％，这是显着不同的。在同一列中，当p <0.05时。采用圆盘扩散法测定种子油的抗菌活性，冷浸提取的种皮和全种子表现出显着的抗菌活性。研究表明，种子油可作为/替代食品的潜在来源，并有望通过分析有用的具有生物活性标记物的种子部位的分析油，用于药物，营养保健品和化妆品。冷热法所有浓度的37％，在同一色谱柱中p <0.05时有显着差异。通过圆盘扩散法测定种子油的抗菌活性，冷浸提取的种皮和全种子显示出显着的抗微生物性-细菌活性。研究表明，种子油可作为/替代食品的潜在来源，并有望通过分析有用的具有生物活性标记物的种子部位的分析油，用于药物，营养保健品和化妆品。冷热法所有浓度的37％，在同一色谱柱中p <0.05时有显着差异。通过圆盘扩散法测定种子油的抗菌活性，冷浸提取的种皮和全种子显示出显着的抗微生物性-细菌活性。研究表明，种子油可作为/替代食品的潜在来源，并有望通过分析有用的具有生物活性标记物的种子部位的分析油，用于药物，营养保健品和化妆品。