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Study of phytochemical content, antioxidant and larvicidal property of different solvent extracts of Clerodendrum infortunatum and Citrus grandis
Indian Journal of Traditional Knowledge ( IF 0.7 ) Pub Date : 2021-05-27
A Swargiary, K Brahma, T Boro, M Daimari, M K Roy

The present study was carried out to investigate the phytochemical, antioxidant and larvicidal property of different solvent extracts of leaves of Clerodendrum infortunatum and fruit peel of Citrus grandis. The antioxidant property was studied by ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), 1,1‑diphenyl‑2‑picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and thiobarbituric acid reactive species (TBARS) assays. The total phenolic and flavonoid contents of the extracts were estimated following standard protocols. Larvicidal activity of the plant extracts were evaluated following standard WHO protocol. In a series of test doses (100 to 2000 μg/mL), 20 numbers of Aedes aegypti larvae were exposed and the mortality was recorded after 24 h and LC50 were calculated. The study showed that the C. grandis extracts have better phytochemical, antioxidant and larvicidal activity compared to C. infortunatum. Among the four solvent fractions, diethyl ether extract showed higher activity in both the plants. The present study thus showed potential larvicidal property of the plants against A. aegypti. However, further characterization and identification of active compound (s) need to be carried out to study the exact mode of action.

中文翻译:

香雪兰和柑桔不同溶剂提取物的植物化学含量,抗氧化剂和杀幼虫特性的研究

本研究旨在研究桔梗叶片和柑桔果皮不同溶剂提取物的植物化学,抗氧化和杀幼虫特性。通过三氧化二铁还原抗氧化能力(FRAP),总抗氧化能力(TAC),1,1-二苯基-2-吡啶甲基酰肼(DPPH),2,2'-叠氮基双-(3-乙基苯并噻唑啉-6-磺酸盐)研究了抗氧化性能(ABTS)和硫代巴比妥酸反应物种(TBARS)分析。提取物的总酚和类黄酮含量是根据标准方案估算的。按照标准的WHO方案评估植物提取物的杀幼虫活性。在一系列测试剂量(100至2000μg/ mL)中,暴露了20个埃及伊蚊幼虫,并在24小时后记录了死亡率并计算了LC50。研究表明,C。与桔梗隐孢子虫相比,格兰迪斯提取物具有更好的植物化学,抗氧化剂和杀幼虫活性。在四种溶剂馏分中,乙醚提取物在两种植物中均显示出较高的活性。因此,本研究表明了该植物对埃及伊蚊的潜在杀幼虫特性。但是,还需要对活性化合物进行进一步的表征和鉴定,以研究确切的作用方式。
更新日期:2021-05-27
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