Amphiphilic Conjugated Polythiophene-based Fluorescence “Turn on” Sensor for Selective Detection of Escherichia coli in Water and Milk,Bulletin of the Korean Chemical Society

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Amphiphilic Conjugated Polythiophene-based Fluorescence “Turn on” Sensor for Selective Detection of Escherichia coli in Water and Milk
Bulletin of the Korean Chemical Society ( IF 2.3 ) Pub Date : 2021-05-26 , DOI: 10.1002/bkcs.12333
Jimin Shim ₁ , Salah M. Tawfik _{1,

2} , Daniel T. Thangadurai ₃ , Yong‐Ill Lee ₁

Affiliation

The ability to detect pathogenic microbes in water and food samples is of prominent interest, but it remains a great challenge. Herein, we report a simple fluorescence sensor based on amphiphilic conjugated polythiophene (ACPT) for the detection of gram-negative pathogenic bacteria, Escherichia coli, in pH 7.0. The ACPT sensor was synthesized by the oxidative polymerization of water-soluble thiophene monomers in an aqueous solution. The ACPT sensor undergoes the fluorescence “turn on” response at 432 nm upon the addition of E. coli. The ionic complex, which is generated by electrostatic interaction during the short incubation time of 15 min, resulting in the enhancement of the fluorescence intensity of ACPT. The application of ACPT enables the detection of E. coli pathogens in tap water and milk samples with good recoveries of 96.94–100.83% and 93.27–116.75%, respectively, and excellent reproducibility (n = 3, relative standard deviation [RSD] = 1.78%). The current findings pave the way for the promising application of a novel ACPT system that could be used in food safety and environmental monitoring.

中文翻译：

用于选择性检测水和牛奶中大肠杆菌的两亲共轭聚噻吩荧光“开启”传感器

检测水和食物样品中病原微生物的能力引起了人们的极大兴趣，但这仍然是一个巨大的挑战。在此，我们报告了一种基于两亲共轭聚噻吩 (ACPT) 的简单荧光传感器，用于检测pH 7.0的革兰氏阴性病原菌大肠杆菌。ACPT 传感器是通过水溶性噻吩单体在水溶液中的氧化聚合合成的。ACPT 传感器在加入大肠杆菌后在 432 nm 处发生荧光“开启”响应。离子复合物，在 15 分钟的短孵育时间内通过静电相互作用产生，导致 ACPT 的荧光强度增强。ACPT的应用使检测自来水和牛奶样品中的大肠杆菌病原体分别具有 96.94–100.83% 和 93.27–116.75% 的良好回收率，以及出色的重现性（n = 3，相对标准偏差 [RSD] = 1.78%）。目前的研究结果为可用于食品安全和环境监测的新型 ACPT 系统的有前景应用铺平了道路。

更新日期：2021-07-27

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