The diversity of the plasmablast signature across species and experimental conditions: A meta-analysis,Immunology

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The diversity of the plasmablast signature across species and experimental conditions: A meta-analysis
Immunology ( IF 4.9 ) Pub Date : 2021-05-26 , DOI: 10.1111/imm.13344
Alexis Grasseau ₁ , Marina Boudigou ₁ , Magalie Michée-Cospolite ₁ , Céline Delaloy ₂ , Olivier Mignen ₁ , Christophe Jamin _{1,

3} , Divi Cornec _{1,

3} , Jacques-Olivier Pers _{1,

3} , Laëtitia Le Pottier ₁ , Sophie Hillion _{1,

3}

Affiliation

Antibody-secreting cells (ASC) are divided into two principal subsets, including the long-lived plasma cell (PC) subset residing in the bone marrow and the short-lived subset, also called plasmablast (PB). PB are described as a proliferating subset circulating through the blood and ending its differentiation in tissues. Due to their inherent heterogeneity, the molecular signature of PB is not fully established. The purpose of this study was to decipher a specific PB signature in humans and mice through a comprehensive meta-analysis of different data sets exploring the PB differentiation in both species and across different experimental conditions. The present study used recent analyses using whole RNA sequencing in prdm1-GFP transgenic mice to define a reliable and accurate PB signature. Next, we performed similar analysis using current data sets obtained from human PB and PC. The PB-specific signature is composed of 155 and 113 genes in mouse and human being, respectively. Although only nine genes are shared between the human and mice PB signature, the loss of B-cell identity such as the down-regulation of PAX5, MS4A1, (CD20) CD22 and IL-4R is a conserved feature across species and across the different experimental conditions. Additionally, we observed that the IRF8 and IRF4 transcription factors have a specific dynamic range of expression in human PB. We thus demonstrated that IRF4/IRF8 intranuclear staining was useful to define PB in vivo and in vitro and able to discriminate between atypical PB populations and transient states.

中文翻译：

跨物种和实验条件的浆母细胞特征的多样性：荟萃分析

抗体分泌细胞 (ASC) 分为两个主要亚群，包括存在于骨髓中的长寿命浆细胞 (PC) 亚群和短寿命亚群，也称为浆母细胞 (PB)。PB 被描述为通过血液循环并结束其在组织中的分化的增殖子集。由于其固有的异质性，PB 的分子特征尚未完全确定。本研究的目的是通过对不同数据集的综合荟萃分析来破译人类和小鼠中特定的 PB 特征，探索两种物种和不同实验条件下的 PB 分化。本研究使用了最近在prdm1中使用全 RNA 测序的分析-GFP 转基因小鼠定义可靠和准确的 PB 特征。接下来，我们使用从人类 PB 和 PC 获得的当前数据集进行了类似的分析。PB 特异性特征分别由小鼠和人类中的 155 个和 113 个基因组成。尽管人类和小鼠 PB 特征之间只有 9 个基因共享，但 B 细胞特性的丧失，例如 PAX5、MS4A1、(CD20) CD22 和 IL-4R 的下调是跨物种和跨不同物种的保守特征。实验条件。此外，我们观察到 IRF8 和 IRF4 转录因子在人类 PB 中具有特定的动态表达范围。因此，我们证明 IRF4/IRF8 核内染色可用于在体内和体外定义 PB，并能够区分非典型 PB 群体和瞬态。

更新日期：2021-05-26

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