ABSTRACT: Urechis unicinctus is an important commercial and ecological invertebrate that has potential applications in the study of marine invertebrate evolution and marine pharmaceutical development. Here we analyzed the intestinal microbial diversity of U. unicinctus from 2 different habitats using 16S rDNA 454 high-throughput sequencing. The dominant phyla were Proteobacteria, Bacterioidetes, Firmicutes, and Actinobacteria in gut samples of U. unicinctus, which significantly differed from those in its 2 habitats (i.e. intertidal mudflat and pond polyculture). Exceptions were Proteobacteria, Firmicutes and Bacterioidetes, which were the dominant phyla in the sediment and water samples. The top 15 genera in the gut samples did not show any significant differences between the 2 habitats. Functional analysis of the intestinal microbial community showed that metabolism, including carbohydrate and amino acid metabolism, was the most important function. Methane metabolism was one of the main components of energy metabolism. The gut microbes also played an important role in environmental and genetic information processing, cellular processes, etc. These findings provide an understanding of gut microbiome composition and diversity in U. unicinctus from 2 different habitats. Our results can inform the management of animal health in U. unicinctus farming activities and further gut microecology research.

中文翻译：

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来自两种不同生境的虾U的肠道微生物多样性和功能分析：日本对虾和沿海地区混养的池塘

摘要：Urechis unicinctus是一种重要的商业和生态无脊椎动物，在海洋无脊椎动物进化和海洋药物开发的研究中具有潜在的应用前景。在这里，我们使用16S rDNA 454高通量测序分析了来自2个不同栖息地的单头U. unicinctus的肠道微生物多样性。在U. unicinctus的肠道样本中，优势菌群是Proteobacteria，Bacterioidetes，Firmicutes和Actinobacteria，这与它的两个栖息地（即潮间带滩涂和池塘混养）明显不同。例外的是变形菌门，厚壁菌门和Bacterioidetes，是沉积物和水样中的优势种。肠道样品中排名前15的属在2个生境之间没有显示任何显着差异。对肠道微生物群落的功能分析表明，代谢（包括碳水化合物和氨基酸代谢）是最重要的功能。甲烷代谢是能量代谢的主要组成部分之一。肠道微生物在环境和遗传信息处理，细胞过程等方面也起着重要作用。这些发现提供了对来自2个不同生境的单毛U鱼肠道微生物组组成和多样性的了解。我们的研究结果可以为U. unicinctus养殖活动中的动物健康管理和进一步的肠道微生态研究提供信息。